RIN1 regulates cell migration through RAB5 GTPases and ABL tyrosine kinases.
RIN1 regulates cell migration through RAB5 GTPases and ABL tyrosine kinases.
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DOI：
10.4161/cib.25421
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发表时间：
2013-09-01
影响因子：
--
通讯作者：
Colicelli J
中科院分区：
文献类型：
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作者：
Balaji K;Colicelli J
刺激受体酪氨酸激酶(RTK)，如EGFR，导致RAS激活，继而RIN1激活。RIN1进而激活RAB5家族GTP酶和ABL酪氨酸激酶。不出所料，RIN1的表达与RAB5介导的EGFR内吞作用直接相关。我们之前的研究表明，正常的受体内吞作用和内化的EGFR命运还取决于RIN1同时激活ABL酪氨酸激酶的能力，这与ABL激酶在细胞骨架重塑中的既定作用以及越来越多的证据表明这种重塑在内吞过程中发挥作用是一致的。在这里，我们报道了生长因子引导的细胞迁移，这是一个涉及受体内吞和肌动蛋白重塑的生理过程，也需要RIN1协调RAB5 GTPase和ABL酪氨酸激酶途径的能力。
Stimulation of a receptor tyrosine kinase (RTK), such as EGFR, leads to RAS activation followed by RIN1 activation. RIN1, in turn, activates RAB5 family GTPases, as well as ABL tyrosine kinases. As expected, RIN1 expression directly correlates with RAB5-mediated EGFR endocytosis. We previously showed that normal receptor endocytosis and internalized EGFR fate also depend on the ability of RIN1 to concomitantly activate ABL tyrosine kinases, consistent with the established role of ABL kinases in cytoskeleton remodeling and the growing evidence that such remodeling plays a role in endocytic processes. Here we report that growth factor-directed cell migration, a physiological process that involves receptor endocytosis and actin remodeling, also requires the ability of RIN1 to coordinate RAB5 GTPase and ABL tyrosine kinase pathways.