Evolutionary modification of cell lineage in the direct-developing sea urchin Heliocidaris erythrogramma.
Evolutionary modification of cell lineage in the direct-developing sea urchin Heliocidaris erythrogramma.
复制标题
直接发育的海胆 Heliocidaris erythrogramma 细胞谱系的进化修饰。
DOI：
10.1016/0012-1606(89)90242-x
复制
发表时间：
1989
影响因子：
2.7
通讯作者：
Raff,RA
中科院分区：
文献类型：
--
作者：
Wray,GA;Raff,RA
海胆Heliocidaris erythrogramma经过直接发育，绕过了通常的海胆幼虫。我们提出了一个分析细胞谱系在H。红细胞数是发育模式中这种进化变化的机制基础的定义的一部分。显微注射荧光示踪染料和表面标记与活体染料用于以下幼虫的命运2细胞，8细胞和16细胞卵裂球，并检查轴向规格。动物-植物轴和成体背腹轴在H中基本上未改变。红纹动物细胞的命运是非常相似的那些典型的发展中的物种，然而，植物细胞的命运在H。红细胞计数显著改变。在8细胞胚胎中，植物性卵裂球的命运之间存在径向差异：背侧植物性卵裂球对外胚层的后代贡献比例更大，对中胚层的后代贡献更小，而腹侧植物性卵裂球在命运上具有互补的偏向性。此外，植物细胞的命运比典型的开发者更多变。H中无细胞。erythrogramma的命运与那些典型的发展echinoids的micromeres和macromeres。相反，16细胞胚胎中的所有植物细胞都可以为外胚层和肠道贡献后代。因此，在直接发育的进化过程中，细胞谱系划分的卵裂模式和时间发生了变化。红纹
The sea urchinHeliocidaris erythrogrammaundergoes direct development, bypassing the usual echinoid pluteus larva. We present an analysis of cell lineage inH. erythrogrammaas part of a definition of the mechanistic basis for this evolutionary change in developmental mode. Microinjection of fluoresceinated tracer dye and surface marking with vital dye are used to follow larval fates of 2-cell, 8-cell, and 16-cell blastomeres, and to examine axial specification. The animal-vegetal axis and adult dorsoventral axis are basically unmodified inH. erythrogramma. Animal cell fates are very similar to those of typically developing species; however, vegetal cell fates inH. erythrogrammaare substantially altered. Radial differences exist among vegetal blastomere fates in the 8-cell embryo: dorsal vegetal blastomeres contribute proportionately more descendants to ectodermal and fewer to mesodermal fates, while ventral vegetal blastomeres have a complementary bias in fates. In addition, vegetal cell fates are more variable than in typical developers. There are no cells inH. erythrogrammawith fates comparable to those of the micromeres and macromeres of typically developing echinoids. Instead, all vegetal cells in the 16-cell embryo can contribute progeny to ectoderm and gut. Alterations have thus arisen in cleavage patterns and timing of cell lineage partitioning during the evolution of direct development inH. erythrogramma.