The sequence and genomic organization of the human type 2 angiotensin II receptor.
The sequence and genomic organization of the human type 2 angiotensin II receptor.
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人类 2 型血管紧张素 II 受体的序列和基因组结构。
DOI：
10.1006/bbrc.1995.1537
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发表时间：
1995
期刊：
影响因子：
--
通讯作者：
Elton,TS
中科院分区：
文献类型：
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作者：
Martin,MM;Elton,TS
以人血管紧张素II型2受体（hAT2R） cDNA为探针，筛选人类基因组DNA文库。分离并鉴定了几个阳性克隆。hAT2R cDNA序列与hAT2R基因组克隆序列的比较表明，hAT2R基因由三个外显子组成，长度至少为5kb。外显子1和2编码5 '未翻译mRNA序列，外显子3包含hAT2R的整个不间断开放阅读框。对hAT2R基因5 ' -侧翼区域的序列分析表明，它包含许多真核生物启动子中发现的典型序列基序。然而，有趣的是，该启动子区域还包括干扰素一致序列结合蛋白位点（ICSBP）和假定的胚胎长末端重复结合蛋白位点（ELF）。这些新的假定的转录因子结合位点的存在表明该基因可能以一种独特的方式受到调节。
A human genomic DNA library was screened utilizing a human angiotensin II type 2 receptor (hAT2R) cDNA as a probe. Several positive clones were isolated and characterized. A comparison of the hAT2R cDNA sequence with the hAT2R genomic clone sequence suggests that the hAT2R gene is composed of three exons and spans at least 5 kb. Exons 1 and 2 encode for 5′ untranslated mRNA sequence and exon 3 harbors the entire uninterrupted open reading frame of the hAT2R. Sequence analysis of the 5′-flanking region of the hAT2R gene demonstrates that it contains the typical sequence motifs found in many eukaryotic promoters. Interestingly, however, this promoter region also includes an interferon consensus sequence binding protein site (ICSBP) and a putative embryonal, long terminal repeat binding protein (ELF) site. The presence of these novel putative transcription factor binding sites suggests that this gene may be regulated in a unique manner.