Parent-collected respiratory specimens - A novel method for respiratory virus and vaccine efficacy research
Parent-collected respiratory specimens - A novel method for respiratory virus and vaccine efficacy research
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DOI：
10.1016/j.vaccine.2008.01.055
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发表时间：
2008-03-28
期刊：
影响因子：
5.5
通讯作者：
Nolan, Terence M.
中科院分区：
文献类型：
--
作者：
Lambert, Stephen B.;Allen, Kelly M.;Nolan, Terence M.
基于人群的呼吸道研究和疫苗有效性研究以前要求当受试者患有急性呼吸道疾病时进行诊所或家庭访视。这种方法可能意味着父母无意中登记了他们的孩子或报告了感兴趣的疾病。我们对234名学龄前儿童进行了一项基于社区的呼吸系统疾病队列研究，使用父母收集的标本。2003年1月至2004年1月期间，从730种已确定的疾病中收集了563份标本，并使用一组呼吸道病毒聚合酶链反应（PCR）检测法对这些标本进行了检测; 409份（73%）对任何病毒呈阳性。当标本由医护人员父母采集时，当它们包括咽拭子时，或者当报告了非常好的采集技术时，标本不太可能呈阳性。从发病到标本采集的延迟长达5天似乎不会影响病毒鉴定的灵敏度，但延迟6天或更长时间并伴有轻微的检测延迟，阳性率会下降。结合每日症状日志填写和PCR检测，父母采集的标本是一种有效且可接受的方法，可用于未来的疫苗有效性研究和其他基于社区的呼吸道病毒研究。(C)2008爱思唯尔有限公司保留所有权利。
Population-based respiratory research and vaccine efficacy studies have previously required clinic or home visits when a subject had an acute respiratory illness. This method may mean parents are unwitting to enrol their child or report an illness of interest. We conducted a community-based cohort study into respiratory illnesses in 234 pre-school aged children using parent-collected specimens. Between January 2003 and January 2004 there were 563 specimens collected from 730 identified illnesses and these were tested using a panel of respiratory virus polymerase chain reaction (PCR) assays; 409 (73%) were positive for any virus. Specimens were not more likely to be positive when collected by a healthcare worker parent, when they included a throat swab, or when a very good collection technique was reported. A delay from illness onset to specimen collection of up to 5 days did not appear to impact on sensitivity of virus identification, but a delay of six or more days with minor delays in testing saw positivity fall. Combined with daily symptom diary completion and PCR testing, parent-collected specimens are an efficient and acceptable method for the conduct of future vaccine efficacy studies and other community-based respiratory virus research. (C) 2008 Elsevier Ltd. All rights reserved.