Horseradish peroxidase-labeled silver/reduced graphene oxide thin film-modified screen-printed electrode for detection of carcinoembryonic antigen
Horseradish peroxidase-labeled silver/reduced graphene oxide thin film-modified screen-printed electrode for detection of carcinoembryonic antigen
复制标题
DOI：
10.1016/j.bios.2015.12.030
复制
发表时间：
2017-03-15
影响因子：
12.6
通讯作者：
Huang, N. M.
中科院分区：
文献类型：
--
作者：
Lee, S. X.;Lim, H. N.;Huang, N. M.
本研究制作了一种一次性使用、结构简单的电化学免疫传感器，用于癌胚抗原的检测。在该方法中，银纳米颗粒（AgNPs）与还原氧化石墨烯（rGO）混合以修饰丝网印刷碳电极（SPE）的表面。首先，通过使用简单的电化学沉积方法制备AgNPs-rGO修饰的SPE。然后将癌胚抗原（CEA）固定在AgNPs-rGO修饰的SPE上，在一抗和辣根过氧化物酶（HRP）标记的二抗之间，制备了一种新型的电化学免疫传感器。与非夹心法相比，该方法的线性范围为0.05- 0.4 μ g mL（-1），检出限为0.042 μ g mL（-1），而该方法的线性范围为0.05- 0.50 μ g mL（-1），检出限为0.035 μ g mL（-1）。该免疫传感器在检测癌胚抗原方面表现出良好的性能，具有简单、快速、成本低的特点。免疫传感器显示出比无酶传感器更高的灵敏度。(C)2015 Elsevier B. V.版权所有。
In this study, a disposable and simple electrochemical immunosensor was fabricated for the detection of carcinoembryonic antigen. In this method, silver nanoparticles (AgNPs) were mixed with reduced graphene oxide (rGO) to modify the surface of screen-printed carbon electrode (SPE). Initially, AgNPs-rGO modified-SPEs were fabricated by using simple electrochemical deposition method. Then the carcinoembryonic antigen (CEA) was immobilized between the primary antibody and horseradish peroxidase (HRP)-conjugated secondary antibody onto AgNPs-rGO modified-SPEs to fabricate a sandwich-type electrochemical immunosensor. The proposed method could detect the CEA with a linear range of 0.05-0.50 mu g mL(-1) and a detection limit down to 0.035 mu g mL(-1) as compared to its non-sandwich counterpart, which yielded a linear range of 0.05-0.4 mu g mL(-1), with a detection limit of 0.042 mu g mL(-1). The immunosensor showed good performance in the detection of carcinoembryonic antigen, exhibiting a simple, rapid and low-cost. The immunosensor showed a higher sensitivity than an enzymeless sensor. (C) 2015 Elsevier B.V. All rights reserved.