THE EFFECTS ON BIOLOGICAL MATERIALS OF FREEZING AND DRYING BY VACUUM SUBLIMATION .2. EFFECT ON INFLUENZA VIRUS
THE EFFECTS ON BIOLOGICAL MATERIALS OF FREEZING AND DRYING BY VACUUM SUBLIMATION .2. EFFECT ON INFLUENZA VIRUS
复制标题
DOI：
10.1084/jem.100.1.89
复制
发表时间：
1954-01-01
影响因子：
15.3
通讯作者：
PINKERTON, H
中科院分区：
文献类型：
--
作者：
GREIFF, D;BLUMENTHAL, H;PINKERTON, H
测定了流感病毒感染尿囊液的感染性滴度。经过各种程序，包括循环缓慢冻结和解冻，以不同的速率冻结，随后在不同的温度下储存。，以不同的速率冻结，随后在不同的温度下脱水。以及不同程度的脱水。这些因素都影响病毒颗粒的存活率。5个冻融循环导致滴度从10-8.6下降到10-0.8，循环2、3和4比循环1和5损失更大。快速冷却至-40[度]C或缓慢冷却至-80或190[度]C不会造成明显的滴度损失，但快速冷却至-40[度]以上或缓慢冷却至温度。高于-80[度]C会造成明显的滴度损失。储存时滴度的损失只发生在温度下。冻干的效果取决于初始处理和脱水温度，初始冷却至-190或80[度]C比初始冷却至更高温度能更好地保存滴度。最有效的升华温度。分别为0和-80[度]。效果最差的是+20[度]C。在-10、-30和-60[度]C下升华的悬浮液滴度损失一般为中等。初步冷却至-80或-190[度]C，随后在-80或0[度]C脱水，滴度没有损失。在0℃条件下，脱水程度对病毒存活有一定影响，但在4小时的升华条件下，脱水程度对病毒存活有一定影响。在0[度]C的温度下，可以完全防止在此温度下储存时滴度损失。根据已知的物理化学现象，如过冷、玻璃化、不同冷冻速度下冰晶大小和形状的变化、不同的酶失活、盐含量的变化，对所观察到的结果提出了可能的解释。，以及能量水平的变化。
The infectivity titer of influenza virus-infected allantoic fluid was detd. after a variety of procedures involving cyclic slow freezing and thawing-freezing at various rates with subsequent storage at different temps., freezing at various rates with subsequent dehydration at various temps. and different degrees of dehydration. All these factors influenced survival rate of virus particles. Five freeze-thaw cycles resulted in a fall in titer from 10-8.6 to 10-0.8, cycles 2, 3, and 4 causing much greater losses than cycles 1 and 5. Rapid cooling to -40[degree]C or slow cooling to -80 or 190[degree]C did not cause significant titer loss, but rapid cooling to temp. above -40[degree] or slow cooling to temps. above -80[degree]C caused definite titer loss. Loss of titer on storage occurred only at temps. above -40[degree]C The effect of lyophilization depends both on preliminary treatment and dehydration temp. Better conservation of titer was obtained after preliminary cooling to -190 or-80[degree]C than preliminary cooling to higher temps. The most effective sublimation temps. were 0 and -80[degree].; the least effective was +20[degree]C. Titer losses in suspensions sublimated at -10, -30, and -60[degree]C were in general intermediate. No loss in titer occurred after preliminary cooling to -80 or -190[degree]C and subsequent dehydration at -80 or 0[degree]C. The degree of dehydration definitely affects survival of virus on storage at 0[degree]C , but sublimation for 4 hrs. at 0[degree]C gave complete protection against titer loss on storage at this temp. Possible explanations of the observations made are suggested, based on known physio-chemical phenomena such as supercooling, vitrification, variations in size and shape of ice crystals with different freezing speeds, differential enzyme inactivation, changes in salt concn., and changes in energy levels.