White Button Mushroom (Agaricus Bisporus) Exhibits Antiproliferative and Proapoptotic Properties and Inhibits Prostate Tumor Growth in Athymic Mice
White Button Mushroom (Agaricus Bisporus) Exhibits Antiproliferative and Proapoptotic Properties and Inhibits Prostate Tumor Growth in Athymic Mice
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DOI：
10.1080/01635580802192866
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发表时间：
2008-01-01
影响因子：
2.9
通讯作者：
Chen, Shiuan
中科院分区：
文献类型：
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作者：
Adams, Lynn S.;Phung, Sheryl;Chen, Shiuan
白色蘑菇是一种广泛食用的食物，含有有益于预防癌症的植物化学物质。本研究旨在探讨白色蘑菇提取物及其主要成分共轭亚油酸（CLA）对前列腺癌细胞株的体外作用及蘑菇提取物对前列腺癌细胞株的体内作用。在所有测试的细胞系中，蘑菇以剂量依赖性方式抑制细胞增殖，并在处理后72小时内诱导细胞凋亡。CLA在体外抑制前列腺癌细胞系的增殖。DU145和PC3前列腺肿瘤的大小和肿瘤细胞增殖减少与蘑菇提取物治疗的裸鼠，而肿瘤细胞凋亡增加相比，配对喂养的控制。肿瘤的微阵列分析发现，与对照组相比，食用蘑菇的小鼠的基因表达发生了显着变化。基因网络分析确定了涉及细胞死亡、生长和增殖、脂质代谢、TCA循环和免疫应答的网络的改变。本研究提供的数据说明了蘑菇提取物中植物化学物质在体外和体内的抗癌潜力，并支持将白色蘑菇作为膳食成分的建议，可能有助于预防男性前列腺癌。
White button mushrooms are a widely consumed food containing phytochemicals beneficial to cancer prevention. The purpose of this research was to evaluate the effects of white button mushroom extract and its major component, conjugated linoleic acid ( CLA) on prostate cancer cell lines in vitro and mushroom extract in vivo. In all cell lines tested, mushroom inhibited cell proliferation in a dose-dependent manner and induced apoptosis within 72 h of treatment. CLA inhibited proliferation in the prostate cancer cell lines in vitro. DU145 and PC3 prostate tumor size and tumor cell proliferation were decreased in nude mice treated with mushroom extract, whereas tumor cell apoptosis was increased compared to pair-fed controls. Microarray analysis of tumors identified significant changes in gene expression in the mushroom-fed mice as compared to controls. Gene network analysis identified alterations in networks involved in cell death, growth and proliferation, lipid metabolism, the TCA cycle and immune response. The data provided by this study illustrate the anticancer potential of phytochemicals in mushroom extract both in vitro and in vivo and supports the recommendation of white button mushroom as a dietary component that may aid in the prevention of prostate cancer in men.