Photosynthetic and physiological analysis of the rice high-chlorophyll mutant (Gc).
Photosynthetic and physiological analysis of the rice high-chlorophyll mutant (Gc).
复制标题
水稻高叶绿素突变体（Gc）的光合及生理分析
DOI：
10.1016/j.plaphy.2012.07.019
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发表时间：
2012-11
期刊：
影响因子：
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通讯作者：
Z. Kang;Guanrong Li;Junli Huang;X. Niu;Hanyan Zou;G. Zang;Yihao Wenwen;Guixue Wang
中科院分区：
文献类型：
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作者：
Z. Kang;Guanrong Li;Junli Huang;X. Niu;Hanyan Zou;G. Zang;Yihao Wenwen;Guixue Wang
Chlorophyll (Chl) molecules are essential for harvesting light energy in photosynthesis. A rice high-chlorophyll mutant (Gc) with significantly increased Chl b was identified previously in Zhenshan 97B (Oryza sativa indica). However, the mechanism underlying this higher Chl b content and its effects on photosynthetic efficiency are still unclear. Immunoblot and blue native polyacrylamide gel electrophoresis (BN-PAGE) with a second dimension electrophoresis followed by the matrix-assisted laser-desorption/ionization time-of-flight (MALDI-TOF) analysis showed that a few core proteins of photosystem I (PSI) and photosystem II (PSII), and light-harvesting complex II (LHCII) proteins were overexpressed in the mutant plants. Remarkable differences in chloroplast ultrastructure were observed between the wild-type and mutant plants, with the latter having more highly stacked and larger grana. Chl florescence analysis demonstrated that Gc had markedly increased quantum efficiency of photosystem II (ΦPSII), photochemical quenching (qP), non-photochemical quenching (qN) and electron transport rate (ETR). This morphological and physiological adaptation might confer a higher photosynthetic capacity in Gc than the wild-type.
