Lipid composition and polyene antibiotic resistance of Candida albicans mutants.
Lipid composition and polyene antibiotic resistance of Candida albicans mutants.
复制标题
白色念珠菌突变体的脂质组成和多烯抗生素耐药性。
DOI：
10.1139/o78-023
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发表时间：
1978
期刊：
影响因子：
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通讯作者：
A. Oehlschlager
中科院分区：
文献类型：
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作者：
A. M. Pierce;H. Pierce;A. M. Unrau;A. Oehlschlager
用n-甲基-n′-硝基-n -亚硝基胍诱变白色念珠菌，获得4个耐多烯突变体C7、E4、C4、D10，并在含制氨抑素的培养基上分离。突变体对两性霉素B、荧光霉素和念珠菌素具有交叉抗性，对4种多烯的抗性依次为C7 < E4 < C4 < D10。分析了突变株和敏感亲本株的游离甾醇、甾醇酯和磷脂脂肪酸。C7的脂质与敏感亲本相同。突变体E4含有lichesterol和其他Δ8-sterols，表明在Δ8→Δ7异构酶上有一个阻断，大部分的甾醇酯部分由4,14-去甲基甾醇组成。抗性最强的突变体C4和D10的生长速率降低，磷脂脂肪酸发生改变，并且缺乏4,14-去甲基甾醇。突变体C4和D10具有相似的脂质组成，24-亚甲基黑甾醇是主要的甾醇，而烟叶油醇含量较少。
Four polyene-resistant mutants (C7, E4, C4, D10) of Candida albicans were derived by mutagenesis with N-methyl-N′-nitro-N-nitrosoguanidine followed by isolation on nystatin-containing medium. The mutants were cross resistant to amphotericin B, lucensomycin, and candicidin and showed the same order of increasing resistance to the four polyenes tested, i.e., C7 < E4 < C4 < D10. Free sterols, sterol esters, and phospholipid fatty acids were analyzed in the mutants and sensitive parent strain. The lipids of C7 were the same as those of the sensitive parent strain. Mutant E4 contained lichesterol and other Δ8-sterols indicating a block at the Δ8→ Δ7 isomerase, and most of the sterol ester fraction consisted of 4,14-desmethyl sterols. The most resistant mutants C4 and D10 had reduced growth rates, alterations in phospholipid fatty acids, and the absence of 4,14-desmethyl sterols. Mutants C4 and D10 had similar lipid compositions with 24-methylenelanosterol as the major sterol and lesser amounts of obtusifoliol ...