Comparison of osteogenic ability of rat mesenchymal stem cells from bone marrow, periosteum, and adipose tissue
Comparison of osteogenic ability of rat mesenchymal stem cells from bone marrow, periosteum, and adipose tissue
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DOI：
10.1007/s00223-008-9112-y
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发表时间：
2008-03-01
影响因子：
4.2
通讯作者：
Ito, Hiromoto
中科院分区：
文献类型：
--
作者：
Hayashi, Ousuke;Katsube, Yoshihiro;Ito, Hiromoto
间充质干细胞（Mesenchymal stem cells，MSCs）存在于多种组织中，可分化为成骨细胞等多种功能细胞。最近，脂肪组织来源的间充质干细胞（AMSC）已被证明可以分化成许多谱系，它们被认为是组织再生的来源。本研究旨在通过体外培养和体内植入实验比较骨髓间充质干细胞（BMSCs）、骨膜间充质干细胞（PMSCs）和AMSCs的成骨分化能力。我们从7周龄的大鼠中收获这些MSC。接种细胞并在原代培养物中培养7天以测定集落形成单位。骨髓间充质干细胞中的细胞分化率最低（P < 0.001）。原代培养后，在成骨分化条件下进行传代培养1周和2周，以检测矿化以及骨特异性蛋白碱性磷酸酶和骨钙素作为成骨标志物。骨髓间充质干细胞和骨髓间充质干细胞具有明显的成骨分化能力（P < 0.001）。对于体内试验，将这些细胞和羟基磷灰石陶瓷的复合物皮下植入同系大鼠中，并在6周后收获。显微计算机断层扫描（CT）和组织学分析表明，新的骨形成检测在复合材料中使用BMSCs和PMSCs，虽然它是很难检测到在其他复合材料。CT分析也显示BMSC复合材料的骨体积大于AMSC复合材料（P < 0.001）。这些结果表明，骨髓间充质干细胞和骨髓间充质干细胞可能是理想的候选人，用于实际的骨组织再生。
Mesenchymal stem cells (MSCs) reside in many types of tissue and are able to differentiate into various functional cells including osteoblasts. Recently, adipose tissue-derived MSCs (AMSCs) have been shown to differentiate into many lineages, and they are considered a source for tissue regeneration. The purpose of this study was to compare the osteogenic differentiation capability of MSCs from bone marrow (BMSCs), MSCs from periosteum (PMSCs), and AMSCs using in vitro culture and in vivo implantation experiments. We harvested these MSCs from 7-week-old rats. The cells were seeded and cultured for 7 days in primary culture to assay a colony-forming unit. The frequency of the unit was the smallest in the BMSCs (P < 0.001). After primary culture, subculture was performed under osteogenic differentiation conditions for 1 and 2 weeks to detect mineralization as well as the bone-specific proteins of alkaline phosphatase and osteocalcin as osteogenic markers. BMSCs and PMSCs showed distinct osteogenic differentiation capability in comparison with other MSCs (P < 0.001). For the in vivo assay, composites of these cells and hydroxyapatite ceramics were subcutaneously implanted into syngeneic rats and harvested after 6 weeks. Micro-computed tomographic (CT) and histological analyses demonstrated that new bone formation was detected in the composites using BMSCs and PMSCs, although it was hard to detect in other composites. The CT analyses also demonstrated that the bone volume of BMSC composites was more than that of AMSC composites (P < 0.001). These results indicate that BMSCs and PMSCs could be ideal candidates for utilization in practical bone tissue regeneration.