Daily rhythmicity of clock gene transcript levels in fast and slow muscle fibers from Chinese perch (Siniperca chuatsi).
Daily rhythmicity of clock gene transcript levels in fast and slow muscle fibers from Chinese perch (Siniperca chuatsi).
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中华鲈鱼（Siniperca chuatsi）快肌纤维和慢肌纤维时钟基因转录水平的日节律性
DOI：
10.1186/s12864-016-3373-z
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发表时间：
2016-12-08
期刊：
影响因子：
4.4
通讯作者：
Chu WY
中科院分区：
文献类型：
--
作者：
Wu P;Li YL;Cheng J;Chen L;Zhu X;Feng ZG;Zhang JS;Chu WY
背景生物钟基因被认为是脊椎动物生物钟的分子核心，直接参与脊椎动物骨骼肌等组织的昼夜节律调控。鱼类肌节由解剖学上分离的快肌纤维和慢肌纤维组成，它们具有不同的代谢和收缩特性。结果研究了鲈鱼慢肌和快肌的分子生物钟（clock、arntl 1/2、cry 1/2/3、cry-dash、npas 2、nr 1d 1/2、per 1/2/3、rorα和timgenes）及其转录水平。在15个时钟基因中，nrld 2和per 3在慢肌中不具有日节律性，cry 2/3和tim在快肌中不具有日节律性。在慢肌中，除ntl 1、nr 1d 1、nr 1d 2和tim外，大多数时钟同源物的最高表达出现在暗期。除nr 1d 2和tim外，其他时钟基因在快肌中的光周期都有一个峰值期。肌源性调节因子（mrf 4和myf 5）、mstnandpncash的昼夜节律表达与两种肌肉中时钟基因的转录模式呈正相关或负相关。两种肌纤维中时钟基因表达模式的差异表明，时钟系统可能在肌肉类型特异性组织的维持和功能中发挥关键作用。
BackgroundClock genes are considered to be the molecular core of biological clock in vertebrates and they are directly involved in the regulation of daily rhythms in vertebrate tissues such as skeletal muscles. Fish myotomes are composed of anatomically segregated fast and slow muscle fibers that possess different metabolic and contractile properties. To date, there is no report on the characterization of the circadian clock system components of slow muscles in fish.ResultsIn the present study, the molecular clock components (clock,arntl1/2,cry1/2/3,cry-dash,npas2,nr1d1/2,per1/2/3,rorαandtimgenes) and their daily transcription levels were characterized in slow and fast muscles of Chinese perch (Siniperca chuatsi). Among the 15 clock genes,nrld2andper3had no daily rhythmicity in slow muscles, andcry2/3andtimdisplayed no daily rhythmicity in fast muscles of the adult fish. In the slow muscles, the highest expression of the most clock paralogs occurred at the dark period exceptarntl1,nr1d1,nr1d2andtim. With the exception ofnr1d2andtim, the other clock genes had an acrophase at the light period in fast muscles. The circadian expression of the myogenic regulatory factors (mrf4andmyf5),mstnandpncashowed either a positive or a negative correlation with the transcription pattern of the clock genes in both types of muscles.ConclusionsIt was the first report to unravel the molecular clock components of the slow and fast muscles in vertebrates. The expressional pattern differences of the clock genes between the two types of muscle fibers suggest that the clock system may play key roles on muscle type-specific tissue maintenance and function.