Selection of peptides with semiconductor binding specificity for directed nanocrystal assembly
Selection of peptides with semiconductor binding specificity for directed nanocrystal assembly
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DOI：
10.1038/35015043
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发表时间：
2000-06-08
期刊：
影响因子：
64.8
通讯作者：
Belcher, AM
中科院分区：
文献类型：
--
作者：
Whaley, SR;English, DS;Belcher, AM
在生物系统中，有机分子对碳酸钙和二氧化硅等无机材料的成核和矿物相，以及微晶和其他纳米级构件组装成生物功能所需的复杂结构具有显著的控制作用(1-4)。这种将纳米级组件组装成受控和复杂结构的能力促使人们加紧努力，开发模仿或利用生物系统中的识别能力和相互作用的组装方法(5-10)。具有特殊价值的方法可以应用于具有有趣的电子或光学性质的材料，但自然进化并未选择用于生物分子与此类材料之间的相互作用。然而，与金属表面和金属氧化物表面结合的选择性有限的多肽已经被成功地选择(10，11)。在这里，我们扩展了这一方法，并表明组合噬菌体展示文库可以用来进化结合到一系列半导体表面的高度特异性的多肽，这取决于我们所使用的结构相似材料的晶体取向和组成。由于电子设备中包含的结构相关材料非常接近，这种多肽可用于各种实际重要材料的受控放置和组装，从而拓宽了自下而上制造方法的范围。
In biological systems, organic molecules exert a remarkable level of control over the nucleation and mineral phase of inorganic materials such as calcium carbonate and silica, and over the assembly of crystallites and other nanoscale building blocks into complex structures required for biological function(1-4). This ability to direct the assembly of nanoscale components into controlled and sophisticated structures has motivated intense efforts to develop assembly methods that mimic or exploit the recognition capabilities and interactions found in biological systems(5-10). Of particular value would be methods that could be applied to materials with interesting electronic or optical properties, but natural evolution has not selected for interactions between biomolecules and such materials. However, peptides with limited selectivity for binding to metal surfaces and metal oxide surfaces have been successfully selected(10,11). Here we extend this approach and show that combinatorial phage-display libraries can be used to evolve peptides that bind to a range of semiconductor surfaces with high specificity, depending on the crystallographic orientation and composition of the structurally similar materials we have used. As electronic devices contain structurally related materials in close proximity, such peptides may rnd use for the controlled placement and assembly of a variety of practically important materials, thus broadening the scope for 'bottom-up' fabrication approaches.