Dorsal-lateral clock neurons modulate consolidation and maintenance of long-term memory in Drosophila.
Dorsal-lateral clock neurons modulate consolidation and maintenance of long-term memory in Drosophila.
复制标题
背外侧时钟神经元调节果蝇长期记忆的巩固和维持。
DOI：
10.1111/gtc.12923
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发表时间：
2022
期刊：
影响因子：
2.1
通讯作者：
Takaomi Sakai
中科院分区：
文献类型：
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作者：
uki Suzuki;Yuto Kurata;Takaomi Sakai
新形成的记忆最初是不稳定的。然而，如果它被巩固到大脑中，巩固的记忆被存储为稳定的长期记忆（LTM）。尽管最近的进展，LTM的分子和细胞机制尚未完全阐明。果蝇，其中各种遗传工具是可用的，已被用来澄清LTM的分子机制。使用果蝇求爱条件反射试验作为记忆范例，我们先前确定了生物钟基因周期（per）在巩固LTM中起着至关重要的作用，这表明表达过的时钟神经元在LTM中起着至关重要的作用。然而，仍然不完全了解哪些时钟神经元对LTM至关重要。在这里，我们表明，背外侧时钟神经元（LNds）在LTM中起着至关重要的作用。使用LNd特异性分裂GAL 4系，我们证实了LNd中突触传递的破坏损害了LTM的维持。另一方面，在LNds中诱导perRNAi或Per的显性阴性转基因损害了LTM巩固。我们的研究结果表明，递质释放和Per功能的LNds参与求偶记忆加工。
A newly formed memory is initially unstable. However, if it is consolidated into the brain, the consolidated memory is stored as stable long‐term memory (LTM). Despite the recent progress, the molecular and cellular mechanisms of LTM have not yet been fully elucidated. The fruitflyDrosophila melanogaster, for which various genetic tools are available, has been used to clarify the molecular mechanisms of LTM. Using theDrosophilacourtship‐conditioning assay as a memory paradigm, we previously identified that the circadian clock geneperiod(per) plays a vital role in consolidating LTM, suggesting thatper‐expressing clock neurons are critically involved in LTM. However, it is still incompletely understood which clock neurons are essential for LTM. Here, we show that dorsal–lateral clock neurons (LNds) play a crucial role in LTM. Using an LNd‐specific split‐GAL4 line, we confirmed that disruption of synaptic transmission in LNds impaired LTM maintenance. On the other hand, induction ofperRNAi or the dominant‐negative transgene of Per in LNds impaired LTM consolidation. Our results reveal that transmitter release and Per function in LNds are involved in courtship memory processing.