Ectopic Expression of OsJAZs Alters Plant Defense and Development.
Ectopic Expression of OsJAZs Alters Plant Defense and Development.
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OsJAZ 的异位表达改变植物防御和发育
DOI：
10.3390/ijms23094581
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发表时间：
2022-04-21
影响因子：
5.6
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中科院分区：
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茉莉酸（JA）信号传导的关键步骤是由F-box蛋白COI1和JAZ转录抑制因子组成的辅受体复合物的配体依赖性组装。这种受体复合物的组装导致蛋白酶体介导的JAZ抑制因子的降解，而JAZ抑制因子反过来结合并抑制MYC转录因子。许多关于JAZs的研究已经在拟南芥中进行，但JAZs在水稻中的功能在很大程度上是未知的。为了系统地揭示OsJAZs的功能，本研究比较了拟南芥中13个OsJAZs异位表达产生的各种表型和12个AtJAZs过表达（OE）系的表型。系统发育分析表明，这25种蛋白可分为3大类。酵母双杂交（Y2H）分析表明，大多数OsJAZ蛋白可以形成同型二聚体或异源二聚体。统计结果表明，OsJAZ OE植株的表型与AtJAZ OE植株在生长发育和免疫方面存在较大差异。例如，与其他JAZ OE植株相比，OsJAZ11 OE植株表现出ja不敏感表型，对Pst DC3000的抗性增强。经JA处理后的OsJAZ11蛋白稳定性强调了该蛋白的特异性功能。本研究旨在从遗传学角度探讨不同JAZ蛋白功能的共性和特点，筛选具有抗病价值的基因。总的来说，本研究结果为进一步分析水稻JAZ家族蛋白的功能提供了见解。
A key step in jasmonic acid (JA) signaling is the ligand-dependent assembly of a coreceptor complex comprising the F-box protein COI1 and JAZ transcriptional repressors. The assembly of this receptor complex results in proteasome-mediated degradation of JAZ repressors, which in turn bind and repress MYC transcription factors. Many studies on JAZs have been performed in Arabidopsis thaliana, but the function of JAZs in rice is largely unknown. To systematically reveal the function of OsJAZs, in this study, we compared the various phenotypes resulting from 13 OsJAZs via ectopic expression in Arabidopsis thaliana and the phenotypes of 12 AtJAZs overexpression (OE) lines. Phylogenetic analysis showed that the 25 proteins could be divided into three major groups. Yeast two-hybrid (Y2H) assays revealed that most OsJAZ proteins could form homodimers or heterodimers. The statistical results showed that the phenotypes of the OsJAZ OE plants were quite different from those of AtJAZ OE plants in terms of plant growth, development, and immunity. As an example, compared with other JAZ OE plants, OsJAZ11 OE plants exhibited a JA-insensitive phenotype and enhanced resistance to Pst DC3000. The protein stability after JA treatment of OsJAZ11 emphasized the specific function of the protein. This study aimed to explore the commonalities and characteristics of different JAZ proteins functions from a genetic perspective, and to screen genes with disease resistance value. Overall, the results of this study provide insights for further functional analysis of rice JAZ family proteins.