DROSOPHILA 230-KD TFIID SUBUNIT, A FUNCTIONAL HOMOLOG OF THE HUMAN CELL-CYCLE GENE-PRODUCT, NEGATIVELY REGULATES DNA-BINDING OF THE TATA BOX-BINDING SUBUNIT OF TFIID
DROSOPHILA 230-KD TFIID SUBUNIT, A FUNCTIONAL HOMOLOG OF THE HUMAN CELL-CYCLE GENE-PRODUCT, NEGATIVELY REGULATES DNA-BINDING OF THE TATA BOX-BINDING SUBUNIT OF TFIID
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DOI：
10.1101/gad.7.6.1033
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发表时间：
1993-06-01
影响因子：
10.5
通讯作者：
NAKATANI, Y
中科院分区：
文献类型：
--
作者：
KOKUBO, T;GONG, DW;NAKATANI, Y
根据纯化蛋白的氨基酸序列，使用简并寡脱氧核苷酸探针分离编码最大 TFIID 亚基 (p230) 的果蝇 cDNA。整个cDNA序列包含一个开放阅读框，编码2068个氨基酸的多肽，对应于计算的232 kD的分子量。推导的氨基酸序列与人类基因 (CCG1) 编码的蛋白质具有很强的序列相似性，该基因涉及细胞周期进展至 G1，表明 p230 可能是细胞周期调节因子的靶标。通过杆状病毒载体在 Sf9 细胞中表达的重组蛋白直接与来自果蝇、人类和酵母的 TFIID（TFIIDtau 或 TBP）的 TATA 盒结合亚基相互作用。令人惊讶的是，重组p230抑制TATA盒结合活性和TFIIDtau的功能，这表明p230与TFIIDtau的相互作用以及其他因素对其的可能调节可能在TFIID功能中发挥重要作用。
A Drosophila cDNA encoding the largest TFIID subunit (p230) was isolated using a degenerate oligodeoxynucleotide probe based on an amino acid sequence of the purified protein. The entire cDNA sequence contains an open reading frame encoding a polypeptide of 2068 amino acids, corresponding to a calculated molecular mass of 232 kD. The deduced amino acid sequence showed a strong sequence similarity with the protein encoded by a human gene (CCG1) implicated in cell cycle progression through G1, suggesting that p230 may be a target for cell cycle regulatory factors. The recombinant protein expressed in Sf9 cells via a baculovirus vector interacts directly with the TATA box-binding subunit of TFIID (TFIIDtau or TBP) from Drosophila, human, and yeast. Surprisingly, recombinant p230 inhibits the TATA box-binding activity and function of TFIIDtau, suggesting that p230 interactions with TFIIDtau, and possible modulations thereof by other factors may play an important role in TFIID function.