Prevention of Staphylococcus aureus biofilm formation and reduction in established biofilm density using a combination of phage K and modified derivatives
Prevention of Staphylococcus aureus biofilm formation and reduction in established biofilm density using a combination of phage K and modified derivatives
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DOI：
10.1111/j.1472-765x.2012.03205.x
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发表时间：
2012-04-01
影响因子：
2.4
通讯作者：
Coffey, A.
中科院分区：
文献类型：
--
作者：
Kelly, D.;McAuliffe, O.;Coffey, A.
目的：研究噬菌体K及其六种修饰衍生物的混合物阻止金黄色葡萄球菌形成生物膜的能力，并降低已建立的生物膜密度。目的：研究噬菌体K及其六种修饰衍生物的混合物阻止金黄色葡萄球菌形成生物膜的能力，并降低已建立的生物膜密度。方法与结果：制备生物发光葡萄球菌。本研究采用金黄色葡萄球菌Xen29菌株，在37℃静态微滴板中培养48 h，证实该菌株具有较强的生物膜形成能力。随后，去除葡萄球菌已形成的生物膜。在24小时、48小时和72小时的时间内对高噬菌体组合的金黄色葡萄球菌Xen29进行了研究。结果表明，这些生物膜以时间依赖性的方式被消除，72小时后生物膜生物量的减少明显大于24-48小时。金黄色葡萄球菌Xen29与噬菌体鸡尾酒在48小时内完全抑制了生物膜的形成，没有出现噬菌体抗性。结论：总的来说，我们的研究结果证明了一种改良噬菌体组合在预防和成功治疗葡萄球菌方面的潜在应用。金黄色葡萄球菌生物膜，与几种抗生素耐药感染有关。研究意义和影响：本研究首次利用噬菌体K成功去除和预防了葡萄球菌的生物膜。葡萄球菌。
Aims: To investigate the ability of a mixture of phage K and six of its modified derivatives to prevent biofilm formation by Staphylococcus aureus and also to reduce the established biofilm density.Aims: To investigate the ability of a mixture of phage K and six of its modified derivatives to prevent biofilm formation by Staphylococcus aureus and also to reduce the established biofilm density.Methods and Results: The bioluminescence-producing Staph. aureus Xen29 strain was used in the study, and incubation of this strain in static microtitre plates at 37 degrees C for 48 h confirmed its strong biofilm-forming capacity. Subsequently, removal of established biofilms of Staph. aureus Xen29 with the hightitre phage combination was investigated over time periods of 24 h, 48 h and 72 h. Results suggested that these biofilms were eliminated in a time-dependant manner, with biofilm biomass reduction significantly greater after 72 h than after 24-48 h. In addition, initial challenge of Staph. aureus Xen29 with the phage cocktail resulted in the complete inhibition of biofilm formation over a 48-h period with no appearance of phage resistance.Conclusions: In general, our findings demonstrate the potential use of a modified phage combination for the prevention and successful treatment of Staph. aureus biofilms, which are implicated in several antibiotic-resistant infections. Significance and Impact of the Study: This study highlights the first use of phage K for the successful removal and prevention of biofilms of Staph. aureus.