Sensor-array for continuous monitoring of biochemicals for bioprocess control
Sensor-array for continuous monitoring of biochemicals for bioprocess control
复制标题
用于连续监测生物化学物质以进行生物过程控制的传感器阵列
DOI：
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发表时间：
2015
期刊：
影响因子：
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通讯作者：
P. Tathireddy
中科院分区：
文献类型：
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作者：
Tram Nguyen;S. Cho;V. Bhola;S. Ko;Rohit Sharma;J. Magda;P. Tathireddy
在生物制药生产中，监测生物工艺参数对于最大限度地提高细胞生长、活力和密度，以及生物产品浓度和产量至关重要。与此同时，一次性技术由于其低资本成本，最小的污染风险和较低的消毒费用而变得流行。我们开发了一种传感器阵列，该阵列利用新型刺激响应水凝胶作为一次性生物反应器中一次性传感器的传感元件。水凝胶对特定分析物的环境浓度变化的反应是用磁性或压力传感器进行传导的。在这里，我们介绍了葡萄糖、乳酸和渗透压水凝胶的最新结果，分别使用压力和磁传感器进行测量。除了这些结果，我们提出了一种新的水凝胶反应转导方法，以及用于生物过程控制的传感器-传感器阵列的设计。
In biopharmaceutical manufacturing, monitoring of bioprocess parameters is very crucial for maximum cell growth, viability and density, as well as biologic product concentration and yield. At the same time, disposable technologies have become popular due to their low capital costs, minimal contamination risk and lower sterilization overhead. We have developed a sensor-array that utilizes novel stimuli-responsive hydrogels intended for use as the sensing elements of disposable sensors in single use bioreactors. The response of hydrogels to changes in environmental concentration of a specific analyte is transduced using a magnetic or pressure sensor. Here we present recent results from glucose, lactate and osmolality hydrogels measured using pressure and magnetic sensors respectively. In addition to these results, we present a novel transduction method for the hydrogel response, along with a design for a sensor-transducer array for use in bioprocess control.