Amino Acid Profiles in Human Tear Fluids Analyzed by High-Performance Liquid Chromatography and Electrospray Ionization Tandem Mass Spectrometry
Amino Acid Profiles in Human Tear Fluids Analyzed by High-Performance Liquid Chromatography and Electrospray Ionization Tandem Mass Spectrometry
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DOI：
10.1016/j.ajo.2010.11.003
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发表时间：
2011-05-01
影响因子：
4.2
通讯作者：
Kinoshita, Shigeru
中科院分区：
文献类型：
--
作者：
Nakatsukasa, Mina;Sotozono, Chie;Kinoshita, Shigeru
目的：鉴定人泪液中的23种氨基酸谱，并评价眼部疾病是否反映了氨基酸谱。设计：实验室调查。方法：我们使用反相高效液相色谱和电喷雾电离串联质谱法对31名健康志愿者泪液中23种氨基酸的浓度和相对组成进行了评估，并与血浆和房水中的浓度和相对组成进行了比较。我们还评估了33名受影响受试者的泪液氨基酸谱。结果：基底撕裂和反射撕裂的氨基酸谱相似，4组健康志愿者（男性、女性、青年和老年人）的氨基酸谱相似。牛磺酸（Tau）和l -谷氨酰胺的绝对浓度在这些泪液中显著占主导地位。泪液中Tau、l -谷氨酸、l -精氨酸（Arg）和瓜氨酸的相对组成显著高于血浆和房水。氨基酸谱的层次聚类分析清楚地区分了严重的眼表疾病和非眼表疾病。与非眼表疾病患者相比，严重眼表疾病患者Tau、l -蛋氨酸和精氨酸的相对组成降低。结论：泪液氨基酸谱不同于血浆和房水氨基酸谱。稳态泪液氨基酸谱可能反映眼表内稳态，观察到的氨基酸变化可能与眼表疾病状况密切相关。[J]中华眼科杂志，2011;51(1):799- 798。(C)爱思唯尔公司2011。版权所有。)
PURPOSE: To identify the 23 amino acid profiles in human tear fluids, and to evaluate whether the ocular disease conditions reflect the amino acid profiles.DESIGN: Laboratory investigation.METHODS: We evaluated the concentrations and relative composition of 23 amino acids in tear fluids obtained from 31 healthy volunteers using reversed-phase high-performance liquid chromatography and electrospray ionization tandem mass spectrometry, and compared them with those in plasma and aqueous humor. We also evaluated the tear-fluid amino acid profiles from 33 affected subjects.RESULTS: The amino acid profiles of the basal tear and reflex tear were found to be similar, and 4 distinct groups of healthy volunteers (male, female, young, and elderly) showed similar profiles. Absolute concentrations of taurine (Tau) and L-glutamine were significantly dominant in these tear fluids. The relative compositions of Tau, L-glutamic acid, L-arginine (Arg), and citrulline in the tear fluid were significantly higher than those in the plasma and aqueous humor. Analysis of the hierarchical clustering of the amino acid profiles clearly distinguished severe ocular surface diseases from non-ocular surface diseases. The relative compositions of Tau, L-methionine, and Arg decreased in severe ocular surface disease subjects compared with non-ocular surface disease subjects.CONCLUSIONS: Tear-fluid amino acid profiles differ from those in plasma and aqueous humor. Steady-state tear-fluid amino acid profiles might reflect ocular-surface homeostasis and the observed changes of amino acids might have a close relation with the disease conditions on the ocular surface. (Am J Ophthalmol 2011;151:799-808. (C) 2011 by Elsevier Inc. All rights reserved.)