Isolation of chloroplast FtsZ and AtpC, and analysis of protein targeting into the complex chloroplast of the haptophyte Pavlova pinguis
Isolation of chloroplast FtsZ and AtpC, and analysis of protein targeting into the complex chloroplast of the haptophyte Pavlova pinguis
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叶绿体 FtsZ 和 AtpC 的分离，以及对 Pavlova pinguis 复合叶绿体的蛋白质靶向分析
DOI：
10.1508/cytologia.75.203
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发表时间：
2010
期刊：
影响因子：
1
通讯作者：
S. Kawano
中科院分区：
文献类型：
--
作者：
Toshikazu Nishikawa;Hiroyuki Kajitani;Mayuko Sato;Y. Mogi;Y. Moriyama;S. Kawano
附着植物是一种丰富的浮游植物，具有4个膜结合叶绿体。这些叶绿体来源于被真核宿主细胞吸收的红色叶绿体。除了计算分析之外，没有以前的工作报道了对附着菌的蛋白质靶向。本研究克隆了巴夫藻叶绿体蛋白AtpC 1和FtsZ基因，并对其进行了分子结构分析。这些蛋白质在N-末端具有二分序列，其由内质网（ER）信号序列和随后的叶绿体转运肽组成。为了证明ER信号序列和叶绿体靶向序列在体内的功能性，我们将预测的ER信号序列、叶绿体转运肽以及AtpC 1和FtsZ的二分序列分别融合到绿色荧光蛋白（sGFP）的N-末端。然后将其引入培养的烟草细胞中。显微镜下观察发现，AtpC 1和FtsZ预测的内质网信号序列具有定位于内质网的能力，并且以下氨基酸在烟草细胞中作为叶绿体转运肽发挥作用。我们的结论是，P. pinguis的AtpC 1和FtsZ在N-末端有一个二分序列，并与其他独立建立的次生内共生藻类的分子结构相同。
Haptophytes are abundant phytoplankton that possess 4 membrane-bound chloroplasts. These chloroplasts originated from a red alga that was taken up by a eukaryotic host cell. No previous work has reported on the protein targeting of haptophytes, except for computational analyses. We isolated the two genes encoding chloroplast proteins AtpC1 and FtsZ from Pavlova pinguis and analyzed their molecular structure. These proteins had a bipartite sequence at the N-terminus, which consisted of an endoplasmic reticulum (ER) signal sequence followed by a chloroplast transit peptide. To demonstrate the functionality of the ER signal sequences and the chloroplast targeting sequences in vivo, we fused the predicted ER signal sequence, chloroplast transit peptide, and bipartite sequence of AtpC1 and FtsZ individually to the N-terminus of green fluorescent protein (sGFP). This was then introduced into cultured tobacco cells. Microscopic observation revealed that the predicted ER signal sequences of AtpC1 and FtsZ had the ability to localize to the ER, and the following amino acids worked as a chloroplast transit peptide in the tobacco cells. We concluded that the AtpC1 and FtsZ of P. pinguis have a bipartite sequence at the N-terminus and a molecular structure in common with other independently established secondarily endosymbiotic algae.
线粒体和红系叶绿体中的两种类型的 FtsZ 蛋白：FtsZ 的重复与内共生有关。
DOI：
--
发表时间：
2004
期刊：
J.Mol.Evol. 58
影响因子：
--
作者：
H.Shiozawa 他17名;Miyagishima et al.
通讯作者：
Miyagishima et al.
影响因子：
9.2
作者：
Patron, Nicola J.;Inagaki, Yuji;Keeling, Patrick J.
通讯作者：
Keeling, Patrick J.