LONG-TERM REGULATION OF PYRUVATE-DEHYDROGENASE KINASE BY HIGH-FAT FEEDING - EXPERIMENTS IN-VIVO AND IN CULTURED CARDIOMYOCYTES
LONG-TERM REGULATION OF PYRUVATE-DEHYDROGENASE KINASE BY HIGH-FAT FEEDING - EXPERIMENTS IN-VIVO AND IN CULTURED CARDIOMYOCYTES
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DOI：
10.1016/0014-5793(93)80864-q
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发表时间：
1993-12-28
期刊：
影响因子：
3.5
通讯作者：
SUGDEN, MC
中科院分区：
文献类型：
--
作者：
ORFALI, KA;FRYER, LGD;SUGDEN, MC
提供高脂饮食(47%的卡路里为脂肪)28天后，心肌PDHa活性显著下降，而成年大鼠分离的线粒体和新鲜制备的心肌细胞的PDH激酶活性显著增加。高脂喂养大鼠血浆胰岛素浓度与对照组无显著差异，但血浆NEFA浓度升高。在培养25h时，脂肪喂养的大鼠心肌细胞的PDH激酶活性显著下降。在培养基中加入正辛酸和DBcAMP可以阻止这种下降，但不是单独添加。讨论了脂肪酸和胰岛素在高脂饮食对心脏PDH激酶活性的长期调节中所起的作用。
The provision of the high-fat diet (47% of calories as fat) for 28 days evoked a significant decline in cardiac PDHa activity, together with marked increases in the activity of PDH kinase measured in isolated mitochondria and freshly-prepared cardiomyocytes from adult rats. Plasma insulin concentrations in fat-fed rats were not significantly different from control, but plasma NEFA concentrations were elevated. PDH kinase activity in cardiomyocytes from fat-fed rats fell substantially in culture (25 h). This decline was prevented by the inclusion of n-octanoate and DBcAMP in combination, but not individually, in the culture medium. The results are discussed in relation to the role for fatty acids and insulin in the long-term modulation of cardiac PDH kinase activity by high-fat Feeding.