An allotetraploid Brassica napus early-flowering mutant has BnaFLC2-regulated flowering.
An allotetraploid Brassica napus early-flowering mutant has BnaFLC2-regulated flowering.
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DOI：
10.1002/jsfa.6254
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发表时间：
2013-12
影响因子：
4.1
通讯作者：
Yong Huang;Ling Jiang;Y. Ruan;W. Shen;Chunlin Liu
中科院分区：
文献类型：
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作者：
Yong Huang;Ling Jiang;Y. Ruan;W. Shen;Chunlin Liu
开花时间是一个重要的农艺性状，甘蓝型油菜品种间存在着广泛的开花时间变异。用甲基磺酸乙酯（EMS）诱导甘蓝型油菜（Brassica napus）获得的早花突变体GX 50，表现出明显的提早从营养生长向生殖生长的转变。结果GX 50植株比对照野生型植株B提前60 d左右开花。油菜XY15在温室条件下的生长情况。细胞学检查显示，GX 50植株早在5周龄就形成花序，6周龄形成花原基，10周龄形成角果，而10周龄的XY 15植株仍处于营养生长阶段。为了阐明GX 50开花表型的分子机制，我们分析了几个关键调控基因的表达。检测所有五种BnaFLC（BnaFLC 1至BnaFLC 5）、BnaFT和BnaSOC 1的表达。有趣的是，GX50中的BnaFLC表达水平低于XY15中的表达水平。在5个BnaFLC中，只有BnaFLC 2的表达模式与GX 50花器官分化的时间相对应。与先前关于BnaFLC抑制BnaFT和BnaSOC 1表达的知识一致，与XY 15相比，在GX 50中观察到BnaFT和BnaSOC 1水平增加。结论BnaFLC 2基因在B发病中起重要作用，而其他BnaFLC基因在B发病中不起重要作用。油菜GX 50花转变。
BACKGROUND Flowering time is an important agronomic trait, and wide variation in flowering time exists among Brassica napus accessions. GX50 early-flowering mutant, induced from Brassica napus by Ethyl Methane Sulfonate (EMS), exhibits a remarkable early transition from vegetative to reproductive growth. RESULTS GX50 plants flowered about 60 days earlier than the control wild-type plant B. napus XY15 under greenhouse conditions. Cytological examination revealed that the GX50 plants form inflorescences as early as from 5 weeks old, flower primordium from 6 weeks old, and siliques from 10 weeks old, whereas 10-week-old XY15 plants are still at vegetative growth stage. To unravel the molecular mechanisms underlying the GX50 flowering phenotype, we analyzed the expression of several key regulatory genes. Expressions of all five BnaFLCs (BnaFLC1 to BnaFLC5), BnaFT and BnaSOC1 were detected. Interestingly, BnaFLCs expression levels were lower in GX50 than those in XY15. Among the five BnaFLCs, only the expression pattern of BnaFLC2 corresponded to the timing of floral organ differentiation in GX50. In agreement with previous knowledge that BnaFLCs repress expression of BnaFT and BnaSOC1, increased levels of BnaFT and BnaSOC1 were observed in GX50 compared with XY15. CONCLUSION BnaFLC2, but not the other BnaFLC genes, plays an important role in B. napus GX50 floral transition.