A Ubiquitous Platform for Bacterial Nanotube Biogenesis
A Ubiquitous Platform for Bacterial Nanotube Biogenesis
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DOI：
10.1016/j.celrep.2019.02.055
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发表时间：
2019-04-09
期刊：
影响因子：
8.8
通讯作者：
Ben-Yehuda, Sigal
中科院分区：
文献类型：
--
作者：
Bhattacharya, Saurabh;Baidya, Amit K.;Ben-Yehuda, Sigal
我们之前已经描述了膜状纳米管的存在，桥接相邻的细菌，促进营养物质，细胞质蛋白质，甚至质粒的细胞间运输，但使其生物起源的组件仍然难以捉摸。在这里，我们揭示了一个分子装置的身份，提供了一个平台，纳米管的生物合成。使用枯草芽孢杆菌（Bs），我们证明了保守的组件的鞭毛输出装置（FliO，FliP，FliQ，FliR，FlhB和FlhA），指定CORE，双重服务于鞭毛和纳米管组件。突变体缺乏CORE基因，但没有其他鞭毛成分，在纳米管生产和相关的细胞间分子运输都有缺陷。在雅阁中，CORE组件位于纳米管出现的位置。删除不同物种的核心建立核心介导的纳米管形成是普遍的。此外，来自不同物种的外源CORE可以恢复缺乏内源CORE的Bs中的纳米管生成和功能。我们的研究结果表明，核心衍生的纳米管是一个无处不在的细胞器，促进整个细菌王国的细胞间分子贸易。
We have previously described the existence of membranous nanotubes, bridging adjacent bacteria, facilitating intercellular trafficking of nutrients, cytoplasmic proteins, and even plasmids, yet components enabling their biogenesis remain elusive. Here we reveal the identity of a molecular apparatus providing a platform for nanotube biogenesis. Using Bacillus subtilis (Bs), we demonstrate that conserved components of the flagellar export apparatus (FliO, FliP, FliQ, FliR, FlhB, and FlhA), designated CORE, dually serve for flagellum and nanotube assembly. Mutants lacking CORE genes, but not other flagellar components, are deficient in both nanotube production and the associated intercellular molecular trafficking. In accord, CORE components are located at sites of nanotube emergence. Deleting COREs of distinct species established that CORE-mediated nanotube formation is widespread. Furthermore, exogenous COREs from diverse species could restore nanotube generation and functionality in Bs lacking endogenous CORE. Our results demonstrate that the CORE-derived nanotube is a ubiquitous organelle that facilitates intercellular molecular trade across the bacterial kingdom.