Purification and ex vivo expansion of postnatal human marrow mesodermal progenitor cells (Retracted article. See vol. 113, pg. 2370, 2009)
Purification and ex vivo expansion of postnatal human marrow mesodermal progenitor cells (Retracted article. See vol. 113, pg. 2370, 2009)
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DOI：
10.1182/blood.v98.9.2615
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发表时间：
2001-11-01
期刊：
影响因子：
20.3
通讯作者：
Verfaillie, CM
中科院分区：
文献类型：
--
作者：
Reyes, M;Lund, T;Verfaillie, CM
据报道，已知产生肢芽中胚层的间充质干细胞也可以在单细胞水平分化为内脏中胚层细胞，并且可以通过临床适用的方法广泛扩增。这些细胞被命名为中胚层祖细胞（MPCs）。通过消耗来自30多个健康人供体的Cp 45（+）/血型糖蛋白-A（GlyA）（+）细胞的骨髓单核细胞来选择MPC。将细胞在含有表皮生长因子和血小板衍生生长因子BB以及2%或更少胎牛血清的纤连蛋白上培养。发现1/5 × 10（3）个CD 45（-）GlyA（-）细胞或1/10（6）个骨髓单个核细胞产生小粘附细胞簇。细胞倍增时间为48至72小时，并且细胞已在培养物中扩增超过60个细胞倍增时间。MPC是CD 34（-）、CD 44（低）、CD 45（-）、CD 117（cKit）（-）、I-HLA（-）和HLA-DR-。MPC分化为肢芽中胚层细胞（成骨细胞、软骨细胞、脂肪细胞、基质细胞和骨骼肌成肌细胞）以及内脏中胚层细胞（内皮细胞）。逆转录病毒标记证实单个MPCs可分化为肢芽细胞和内脏中胚层细胞。因此，在临床适用条件下增殖而没有明显衰老并且在单细胞水平上不仅分化成间充质细胞而且分化成内脏中胚层细胞的MPC可能是用于治疗影响中胚层起源细胞的遗传或退行性疾病的干细胞的理想来源。(C)2001年，美国血液学会。
It is here reported that mesenchymal stem cells known to give rise to limb-bud mesoderm can, at the single-cell level, also differentiate into cells of visceral mesoderm and can be expanded extensively by means of clinically applicable methods. These cells were named mesodermal progenitor cells (MPCs). MPCs were selected by depleting bone marrow mononuclear cells from more than 30 healthy human donors of Cp45(+)/glycophorin-A (GlyA)(+) cells. Cells were cultured on fibronectin with epidermal growth factor and platelet-derived growth factor BB and 2% or less fetal calf serum. It was found that 1/5 x 10(3) CD45(-)GlyA(-) cells, or 1/10(6) bone marrow mononuclear cells, gave rise to clusters of small adherent cells. Cell-doubling time was 48 to 72 hours, and cells have been expanded in culture for more than 60 cell doublings. MPCs are CD34(-), CD44(low), CD45(-), CD117 (cKit)(-), class I-HLA(-), and HLA-DR-. MPCs differentiated into cells of limb-bud mesoderm (osteoblasts, chondrocytes, adipocytes, stroma cells, and skeletal myoblasts) as well as visceral mesoderm (endothelial cells). Retroviral marking was used to definitively prove that single MPCs can differentiate into cells of limb bud and visceral mesoderm. Thus, MPCs that proliferate without obvious senescence under clinically applicable conditions and differentiate at the single-cell level not only into mesenchymal cells but also cells of visceral mesoderm may be an ideal source of stem cells for treatment of genetic or degenerative disorders affecting cells of mesodermal origin. (C) 2001 by The American Society of Hematology.