The protective effect of berberine against neuronal damage by inhibiting matrix metalloproteinase-9 and laminin degradation in experimental autoimmune encephalomyelitis
The protective effect of berberine against neuronal damage by inhibiting matrix metalloproteinase-9 and laminin degradation in experimental autoimmune encephalomyelitis
复制标题
小檗碱抑制基质金属蛋白酶9和层粘连蛋白降解对实验性自身免疫性脑脊髓炎神经元损伤的保护作用
DOI：
10.1179/1743132812y.0000000156
复制
发表时间：
2013-05-01
影响因子：
1.9
通讯作者：
Chen, Xiaohong
中科院分区：
文献类型：
--
作者：
Jiang, Ying;Wu, Aiming;Chen, Xiaohong
Abstract Objective: This study aims to assess the protective effect of berberine against neuronal damage in the brain parenchyma of mice with experimental autoimmune encephalomyelitis (EAE). Methods: EAE was induced in female C57 BL/6 mice with myelin oligodendrocyte glycoprotein 35–55 amino acid peptide. The berberine treatment was initiated on the day of disease onset and administered daily until the mice were sacrificed. Terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling (TUNEL) assay, gelatin gel, and gelatin in situ zymography were analysed in this study. Results: Berberine reduced the TUNEL-positive neuronal cells of EAE mice. Gelatin gel and gelatin in situ zymography showed up-regulation of gelatinase activity, which was mainly located in neurons and colocalized with remarkable laminin degradation in EAE mice. Berberine significantly inhibited gelatinase activity and reduced the laminin degradation in EAE mice. Discussion: Our data suggest that berberine could provide protection against neuronal damage in EAE by inhibiting gelatinase activity and reducing laminin degradation. These findings provide further support that berberine can be a potential therapeutic agent for multiple sclerosis.
