Genomes and virulence factors of novel bacterial pathogens causing bleaching disease in the marine red alga Delisea pulchra.
Genomes and virulence factors of novel bacterial pathogens causing bleaching disease in the marine red alga Delisea pulchra.
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DOI：
10.1371/journal.pone.0027387
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发表时间：
2011
期刊：
影响因子：
3.7
通讯作者：
Thomas T
中科院分区：
文献类型：
--
作者：
Fernandes N;Case RJ;Longford SR;Seyedsayamdost MR;Steinberg PD;Kjelleberg S;Thomas T
Nautella sp. R11是海洋玫瑰杆菌分支的一员，它会引起温带海洋红色大藻Delisea pulchra的白化病。为了开始阐明Nautella sp. R11定植、入侵和诱导pulchra d.p ulchra漂白的分子机制，我们对其基因组进行了测序和分析。基因组编码了几个因素，如粘附机制、藻类代谢物运输系统、赋予氧化应激抗性的酶、细胞溶解素和可能允许Nautella sp. R11转变为致病性生活方式的全球调节机制。在R11基因组中也发现了许多常见的植物致病性细菌的毒力效应物，如植物激素吲哚乙酸、纤维素原纤维、琥珀聚糖和结瘤蛋白L.与非致病性玫瑰杆菌菌株和新发现的病原体Phaeobacter sp. LSS9的比较基因组显示，所有基因组中推测的毒力因子呈斑块状分布。还鉴定出致病玫瑰杆菌菌株特有的群体感应（QS）依赖性转录调节因子。这一观察结果支持了一个模型，即毒力因素和依赖于qs的调节机制的结合，使宿主藻类的附生微生物群落的本地成员能够切换到一种致病的生活方式，特别是在先天宿主防御机制受损的环境条件下。
Nautella sp. R11, a member of the marine Roseobacter clade, causes a bleaching disease in the temperate-marine red macroalga, Delisea pulchra. To begin to elucidate the molecular mechanisms underpinning the ability of Nautella sp. R11 to colonize, invade and induce bleaching of D. pulchra, we sequenced and analyzed its genome. The genome encodes several factors such as adhesion mechanisms, systems for the transport of algal metabolites, enzymes that confer resistance to oxidative stress, cytolysins, and global regulatory mechanisms that may allow for the switch of Nautella sp. R11 to a pathogenic lifestyle. Many virulence effectors common in phytopathogenic bacteria are also found in the R11 genome, such as the plant hormone indole acetic acid, cellulose fibrils, succinoglycan and nodulation protein L. Comparative genomics with non-pathogenic Roseobacter strains and a newly identified pathogen, Phaeobacter sp. LSS9, revealed a patchy distribution of putative virulence factors in all genomes, but also led to the identification of a quorum sensing (QS) dependent transcriptional regulator that was unique to pathogenic Roseobacter strains. This observation supports the model that a combination of virulence factors and QS-dependent regulatory mechanisms enables indigenous members of the host alga's epiphytic microbial community to switch to a pathogenic lifestyle, especially under environmental conditions when innate host defence mechanisms are compromised.
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影响因子：
5.1
作者：
Case, Rebecca J.;Longford, Sharon R.;Kjelleberg, Staffan
通讯作者：
Kjelleberg, Staffan
影响因子：
2.2
作者：
Amaro, AM;Fuentes, MS;Suárez-Isla, BA
通讯作者：
Suárez-Isla, BA
影响因子：
4.4
作者：
Ashen, JB;Goff, LJ
通讯作者：
Goff, LJ
影响因子：
4.4
作者：
Banin, E;Khare, SK;Rosenberg, E
通讯作者：
Rosenberg, E
DOI：
10.1073/pnas.0308052101
发表时间：
2004-04-06
影响因子：
11.1
作者：
Butler, SM;Camilli, A
通讯作者：
Camilli, A