KEY MORPHOLOGICAL FEATURES OF APOPTOSIS MAY OCCUR IN THE ABSENCE OF INTERNUCLEOSOMAL DNA FRAGMENTATION
KEY MORPHOLOGICAL FEATURES OF APOPTOSIS MAY OCCUR IN THE ABSENCE OF INTERNUCLEOSOMAL DNA FRAGMENTATION
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DOI：
10.1042/bj2860331
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发表时间：
1992-09-01
影响因子：
4.1
通讯作者：
SKILLETER, DN
中科院分区：
文献类型：
--
作者：
COHEN, GM;SUN, XM;SKILLETER, DN
细胞凋亡是细胞死亡的一种主要形式。其特征在于染色质浓缩、细胞体积减小和核酸内切酶将DNA切割成寡核小体长度片段。凝胶电泳检测这些片段，作为DNA梯状条带，目前被用作细胞凋亡的主要生化指标。在这里，我们报告，细胞凋亡的关键形态学变化可以从实验中分离核酸内切酶活性产生的DNA片段。因此，DNA的核小体间裂解可能是凋亡过程中的后期事件。
Apoptosis, a major form of cell death. is characterized by chromatin condensation, a reduction in cell volume and endonuclease cleavage of DNA into oligonucleosomal length fragments. The detection of these fragments by gel electrophoresis, as a DNA ladder, is currently used as the major biochemical index of apoptosis. Here we report that key morphological changes of apoptosis can be dissociated experimentally from the DNA fragmentation produced by endonuclease activity. Internucleosomal cleavage of DNA is thus likely to be a later event in the apoptotic process.