Complete Genome Sequence of a Chinese Isolate of Potato virus X and Analysis of Genetic Diversity
Complete Genome Sequence of a Chinese Isolate of Potato virus X and Analysis of Genetic Diversity
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DOI：
10.1111/j.1439-0434.2007.01365.x
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发表时间：
2008-06
影响因子：
1.5
通讯作者：
X. Yu;Hongwei Wang;Y. Lan;Xicun Zhu;X. -. Li;Z. Fan;H. Li;Y. Wang
中科院分区：
文献类型：
--
作者：
X. Yu;Hongwei Wang;Y. Lan;Xicun Zhu;X. -. Li;Z. Fan;H. Li;Y. Wang
从三个重叠的cDNA克隆中测定了中国马铃薯病毒X分离物FX21 （PVX-FX21）的全基因组序列。PVX-FX21的基因组长度为6435个核苷酸（不包括poly(A)尾），包含5个开放阅读框（orf）。其全基因组序列与亚洲和欧洲分离株的同源性为95.2-96.3%，与美洲分离株的同源性为77.3-77.8%。完整基因组序列的系统发育分析揭示了两组：欧亚组和美洲组。PVX-FX21属于欧亚群，与三个亚洲分离株形成一个单独的分支。对37株PVX分离株外壳蛋白基因的类似分析也揭示了两个主要群体。来自亚洲的所有PVX分离株聚集在第一类，而来自欧洲和美洲的分离株聚集在这两类。核苷酸序列多样性分析表明，PVX分离株之间不存在地理差异，对编码RNA依赖RNA聚合酶的ORF的限制远高于其他四种ORF。
The complete genomic sequence of a Chinese Potato virus X isolate FX21 (PVX-FX21) was determined from three overlapping cDNA clones. The genome of PVX-FX21 is 6435 nucleotides in length excluding the poly(A) tail and contains five open reading frames (ORFs). Its entire genomic sequence shares 95.2-96.3% identities with Asian and European isolates, and 77.3-77.8% with American isolates. Phylogenetic analysis of the complete genomic sequence reveals two groups: the Eurasian group and the American group. PVX-FX21 belongs to the Eurasian group and forms a separate sub-branch with three Asian isolates. Similar analyses of the coat protein genes of 37 PVX isolates also reveal two major groups. All PVX isolates from Asia are clustered to group I, whereas isolates from Europe and America are clustered to both groups. Nucleotide sequence diversity analyses show that there is no geographical differentiation between PVX isolates and that constraint on the ORF encoding RNA-dependent RNA polymerase is much higher than those on the other four ORFs.