RNA structure drives interaction with proteins
RNA structure drives interaction with proteins
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DOI：
10.1038/s41467-019-10923-5
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发表时间：
2019-07-19
影响因子：
16.6
通讯作者：
Tartaglia, Gian Gaetano
中科院分区：
文献类型：
--
作者：
de Groot, Natalia Sanchez;Armaos, Alexandros;Tartaglia, Gian Gaetano
高通量测序和体内交联方法的结合导致细胞转录组和蛋白质组之间复杂的相互依赖关系的逐渐揭示。然而，控制蛋白质- rna网络相互作用的分子决定因素尚未得到很好的理解。在这里，我们研究了RNA的结构与其与蛋白质相互作用的能力之间的关系。通过计算机、体外和体内实验分析，我们发现RNA中双链区域的数量与蛋白质接触的数量相关。这种关系——我们称之为结构驱动的蛋白质相互作用——允许RNA类型的分类，在基因调控中发挥作用，并可能对相分离核糖核蛋白组装的形成产生影响。我们通过证明高度结构化的RNA可以重新排列蛋白质聚集体的组成来验证我们的假设。我们报告了蛋白质相分离的倾向通过与特定rna的相互作用而减少。
The combination of high-throughput sequencing and in vivo crosslinking approaches leads to the progressive uncovering of the complex interdependence between cellular transcriptome and proteome. Yet, the molecular determinants governing interactions in protein-RNA networks are not well understood. Here we investigated the relationship between the structure of an RNA and its ability to interact with proteins. Analysing in silico, in vitro and in vivo experiments, we find that the amount of double-stranded regions in an RNA correlates with the number of protein contacts. This relationship -which we call structure-driven protein interactivity- allows classification of RNA types, plays a role in gene regulation and could have implications for the formation of phase-separated ribonucleoprotein assemblies. We validate our hypothesis by showing that a highly structured RNA can rearrange the composition of a protein aggregate. We report that the tendency of proteins to phase-separate is reduced by interactions with specific RNAs.