T7 vectors with a modified T7lac promoter for expression of proteins in Escherichia coli
T7 vectors with a modified T7lac promoter for expression of proteins in Escherichia coli
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DOI：
10.1006/abio.1996.0187
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发表时间：
1996-05-01
影响因子：
2.9
通讯作者：
Kaariainen, L
中科院分区：
文献类型：
--
作者：
Peranen, J;Rikkonen, M;Kaariainen, L
PA-糖链用线性梯度的铵（Tokyo）90，407-414洗脱。乙酸盐，pH 9，以1.0 ml/min的流速从0至0.2 M洗脱45 min。箭头表示标准PA-糖7的洗脱位置。Kuraya，N.，和Hase，S.等人（1992）生物化学杂志（J. Biochem.（Tokyo）112，122-链：1，Galb 1 -3GalNAc-PA; 2，Neurons 2 - 3Galb 1 -3GalNAc-PA; 126. 3、Neuron2 - 3Galb 1 -3（Neuron2 -6）GalNAc-PA。8.野村，H.，今关岛Oh-eda，M.，久保田，N.，Tamura，M.，
PA-sugar chains were eluted with a linear gradient of ammonium (Tokyo) 90, 407–414. acetate, pH 9, from 0 to 0.2 M for 45 min at a flow rate of 1.0 ml/min. Arrowheads indicate the elution positions of standard PA-sugar 7. Kuraya, N., and Hase, S.(1992) J. Biochem.(Tokyo) 112, 122–chains: 1, Galb1–3GalNAc-PA; 2, NeuAca2–3Galb1–3GalNAc-PA; 126. 3, NeuAca2–3Galb1–3 (NeuAca2–6) GalNAc-PA. 8. Nomura, H., Imazeki, I., Oh-eda, M., Kubota, N., Tamura, M.,