Fractionation of orange peel phenols in ultrafiltered molasses and mass balance studies of their antioxidant levels
Fractionation of orange peel phenols in ultrafiltered molasses and mass balance studies of their antioxidant levels
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DOI：
10.1021/jf049083j
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发表时间：
2004-12-15
影响因子：
6.1
通讯作者：
Manthey, JA
中科院分区：
文献类型：
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作者：
Manthey, JA
橙子皮糖蜜是果汁生产的副产品，含有高浓度的酚类，包括大量的黄烷酮和黄酮糖苷、多甲氧基化黄酮、羟基肉桂酸酯和其它混杂的酚糖苷和胺。通过吸附、离子交换和尺寸排阻色谱法实现了这些酚的广泛分级。尺寸排阻色谱法有效地分离了超滤糖蜜中的不同类别的黄酮类化合物，包括多甲氧基黄酮，黄烷酮-O-三羟甲基，黄烷酮-和黄酮-O-二糖，最后，黄酮-C-糖苷。早期洗脱类黄酮馏分的大小排阻柱的质谱分析揭示了一个广泛的收集小发生的黄酮苷，其中包括，部分，苷的柠檬苦苷，柠檬醇，和chrysoeriol。大多数羟基肉桂酸在糖蜜回收离子交换色谱法，这也有利于回收馏分含有许多其他杂项酚。总抗氧化剂水平和总酚含量测定糖蜜中的酚类的单独类别。抑制超氧阴离子还原氮蓝四唑表明，显着量的总抗氧化活性在橙子皮糖蜜是由于未成年人发生黄酮。糖蜜中总酚含量的大部分存在于含杂酚的馏分中，也构成了超滤糖蜜中总抗氧化剂的主要部分。
Orange peel molasses, a byproduct of juice production, contains high concentrations of phenols, including numerous flavanone and flavone glycosides, polymethoxylated flavones, hydroxycinnamates, and other miscellaneous phenolic glycosides and amines. Extensive fractionation of these phenols was achieved by adsorption, ion exchange, and size exclusion chromatography. Size exclusion chromatography effectively separated the different classes of flavonoids in ultrafiltered molasses, including the polymethoxylated flavones, flavanone-O-trisaccharides, flavanone- and flavone-O-disaccharides, and, finally, flavone-C-glycosides. Mass spectral analysis of the early-eluting flavonoid fractions off the size exclusion column revealed a broad collection of minor-occurring flavone glycosides, which included, in part, glycosides of limocitrin, limocitrol, and chrysoeriol. Most hydroxycinnamates in the molasses were recovered by ion exchange chromatography, which also facilitated the recovery of fractions containing many other miscellaneous phenols. Total antioxidant levels and total phenolic contents were measured for the separate categories of phenols in the molasses. Inhibition of the superoxide anion reduction of nitroblue tetrazolium showed that a significant amount of the total antioxidant activity in orange peel molasses was attributable to minor-occurring flavones. The miscellaneous phenolic-containing fractions, in which a large portion of the total phenolic content in molasses occurred, also constituted a major portion of the total antioxidants in ultrafiltered molasses.