PHOSPHATASES OF EPIPHYSEAL CARTILAGE STUDIED BY ELECTRON MICROSCOPIC CYTOCHEMICAL METHODS
PHOSPHATASES OF EPIPHYSEAL CARTILAGE STUDIED BY ELECTRON MICROSCOPIC CYTOCHEMICAL METHODS
复制标题
电子显微细胞化学方法研究骨骺软骨磷酸酶
DOI：
--
复制
发表时间：
1971
影响因子：
3.2
通讯作者：
H. Anderson
中科院分区：
文献类型：
--
作者：
T. Matsuzawa;H. Anderson
用电镜细胞化学方法研究了幼年小鼠骺板内碱性磷酸酶、三磷酸腺苷酶（ATPase）和酸性磷酸酶活性的存在和分布。碱性磷酸酶和ATP酶活性与软骨细胞的质膜和基质囊泡的包被膜有关。这些囊泡含有最早被识别的羟基磷灰石沉积物，并可能通过主动过程促进钙化。碱性磷酸酶和ATP酶活性在骨骺的肥大区最大，这是一个开始钙化的区域。酸性磷酸酶活性在软骨细胞的细胞质中是可证明的，与致密体相关联，致密体大于基质囊泡，并且缺乏碱性磷酸酶和ATP酶活性。这些细胞质溶酶体样小体在肥大区数量略多，但在软骨细胞变性和基质钙化的下层区域消失。我们的观察结果不支持以前的建议，由其他人，基质囊泡代表溶酶体。ATP酶和碱性磷酸酶的存在与基质囊泡中的酶促钙和/或磷酸盐浓缩机制相容。
The presence and distribution of alkaline phosphatase, adenosine triphosphatase (ATPase) and acid phosphatase activities in the epiphyseal plates of young mice were studied by electron microscopic cytochemical methods. Alkaline phosphatase and ATPase activities were associated with the plasma membranes of chondrocytes and with the investing membranes of matrix vesicles. These vesicles contain the earliest recognized deposits of hydroxyapatite and may promote calcification through an active process. Alkaline phosphatase and ATPase activities were greatest in the hypertrophic zone of the epiphysis, which is an area of beginning calcification. Acid phosphatase activity was demonstrable in the cytoplasm of chondrocytes in association with dense bodies which were larger than matrix vesicles and were devoid of alkaline phosphatase and ATPase activities. These cytoplasmic lysosome-like bodies were slightly more numerous in the hypertrophic zone but disappeared in the underlying zone of chondrocyte degeneration and matrix calcification. Our observations do not support a previous suggestion by others that matrix vesicles represent lysosomes. The presence of ATPase and alkaline phosphatase is compatible with an enzymatic calcium- and/or phosphate-concentrating mechanism in the matrix vesicles.