A PHYSIOLOGICAL AND MORPHOLOGICAL-STUDY OF THE HORIZONTAL CELL-TYPES OF THE RABBIT RETINA
A PHYSIOLOGICAL AND MORPHOLOGICAL-STUDY OF THE HORIZONTAL CELL-TYPES OF THE RABBIT RETINA
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DOI：
10.1002/cne.902080306
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发表时间：
1982-01-01
影响因子：
2.5
通讯作者：
MILLER, RF
中科院分区：
文献类型：
--
作者：
BLOOMFIELD, SA;MILLER, RF
采用兔视网膜-眼杯灌注分离制备，观察水平细胞的生理和形态。通过细胞内记录技术对神经元进行生理表征，随后用细胞内离子电泳注入辣根过氧化物酶进行形态学鉴定。根据响应波形、振幅强度特性和面积求和特性的变化，对兔水平细胞记录的三种类型进行了区分。这三种类型与无轴突的a型水平细胞和b型水平细胞的体细胞和轴突末梢（各自表现出独特的生理特征）明确相关，这是在使用高尔基浸渍技术的研究中首次描述的（Fisher et Boycott, 1974）。a型水平细胞的大小与其离视盘的视网膜偏心率有直接关系。我们发现了一种独特的A型细胞亚类（细长型或Ae型），它在这里研究的所有细胞中显示出最大的树突场，但在视盘的1.4 mm范围内具有最小的偏心。细长的a型细胞具有与视条纹平行的长而不对称的树突场。这些细胞独特的不对称性和定向表明它们可能具有定向偏倚的感受野特性。生理证据的取向偏向水平细胞提出了支持这一概念。
A perfused, isolated retina-eyecup preparation of the rabbit was utilized to correlate the physiology and morphology of horizontal cells. Neurons were physiologically characterized by intracellular recording techniques and subsequently stained with intracellular iontophoretically injected horseradish peroxidase for morphological identification. Three types of rabbit horizontal cell recordings were differentiated, based on variations in response waveform, amplitude-intensity properties, and area summation characteristics. These 3 types were unequivocally associated with the axonless A-type horizontal cells and the somatic and axon terminal endings (each displaying its own distinct physiology) of B-type horizontal cells first described in studies using Golgi-impregnation techniques (Fisher et Boycott, 1974). The sizes of A-type horizontal cells were directly related to their retinal eccentricities from the optic disk. A unique subclass of A-type cells were discovered (elongated or Ae type) which displayed the largest dendritic field of any cells studied here, yet had the smallest eccentricities, within 1.4 mm of the optic disk. Elongated A-type cells exhibited long asymmetrical dendritic fields which were oriented parallel with the visual streak. The unique asymmetry and orientation of these cells suggests that they may have orientation-biased receptive field properties. Physiological evidence for an orientation-biased horizontal cell is presented in support of this notion.