Efficient vaccine against the virus causing a lethal disease in cultured Cyprinus carpio
Efficient vaccine against the virus causing a lethal disease in cultured Cyprinus carpio
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DOI：
10.1016/s0264-410x(03)00523-1
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发表时间：
2003-12-01
期刊：
影响因子：
5.5
通讯作者：
Kotler, M
中科院分区：
文献类型：
--
作者：
Ronen, A;Perelberg, A;Kotler, M
我们已经分离到一种病毒，它会导致世界上许多国家的观赏锦鲤和鲤鱼（Cyprinus carpio）的致命疾病。这种未分类的病毒，引起肾炎和鳃坏死，因此被命名为鲤鱼肾炎和鳃坏死病毒（CNGV），其形态类似于疱疹病毒，但携带约250-300 kbp的基因组DNA。到目前为止，我们和其他人都无法找到与已知DNA病毒具有显著相似性的CNGV-DNA序列。当水温在18至25摄氏度（允许温度）之间时，该病毒会诱发致命疾病。在这份报告中，我们证明，鲤鱼，暴露于病毒在23 degreesC的3-5天，然后转移到非允许的温度30 degreesC，成为抵抗挑战感染和他们的血清中表现出高水平的病毒特异性抗体。我们已经分离出减毒的非致病性病毒，使病毒接种鲤鱼对疾病的抵抗力。此外，接种疫苗的鱼产生了高水平的抗病毒抗体。因此，我们建议，这种减毒病毒可用作活疫苗，用于根除许多国家的常见和观赏鲤鱼渔业的致命疾病。(C)2003 Elsevier Ltd.保留所有权利。
We have isolated a virus, which causes a mortal disease in cultured ornamental Koi and Common carps (Cyprinus carpio) in many countries worldwide. This unclassified virus, which causes nephritis and gill necrosis, and so has been given the name carp nephritis and gill necrosis virus (CNGV), has a morphology resembling the herpes virus, but bears a genomic DNA of ca 250-300 kbp. So far, both others and we have been unable to find CNGV-DNA sequences possessing a significant similarity to known DNA viruses. The virus induces a lethal disease when water temperature ranges between 18 and 25 degreesC (permissive temperature). In this report, we demonstrate that carps, exposed to the virus at 23 degreesC for 3-5 days and then transferred to the non-permissive temperature of 30 degreesC, became resistant to a challenged infection and their sera demonstrated a high level of virus-specific antibodies. We have isolated attenuated non-pathogenic viruses that render virus-vaccinated carps resistant to the disease. Furthermore, vaccinated fish developed high levels of antibodies against the virus. We suggest, therefore, that this attenuated virus could be used as a live vaccine for the eradication of the mortal disease afflicting Common and ornamental carp fisheries in many countries. (C) 2003 Elsevier Ltd. All rights reserved.