MicroRNA-193b acts as a tumor suppressor gene in human esophageal squamous cell carcinoma via target regulation of KRAS
MicroRNA-193b acts as a tumor suppressor gene in human esophageal squamous cell carcinoma via target regulation of KRAS
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MicroRNA-193b通过KRAS靶点调控作为人食管鳞癌的抑癌基因
DOI：
10.3892/ol.2019.10039
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发表时间：
2019-04-01
期刊：
影响因子：
2.9
通讯作者：
Li, Peng
中科院分区：
文献类型：
--
作者：
Kang, Min;Li, Yan;Li, Peng
近年来，microRNA-193 b（miR-193 b）被认为是多种癌症发生和发展的肿瘤抑制因子。多项研究表明，胰腺癌细胞中的KRAS可受到miR-193 b的调控。然而，miR-193 b在人食管鳞状细胞癌中的功能尚未得到深入研究。本文主要在食管鳞癌细胞中探讨miR-193 b与KRAS的关系。在本研究中，miR-193 b和KRAS在人食管癌细胞和组织中的表达水平进行了评估。双荧光素酶法检测miR-193 b与KRAS的直接调控关系。本文进一步检测了miR-193 b过表达和抑制剂对细胞增殖、迁移/侵袭和凋亡的影响。结果表明，miR-193 b在食管癌组织中的表达明显低于癌旁组织。miR-193 b/KRAS在食管癌组织中的表达水平与肿瘤分期有关。KRAS是miR-193 b的直接靶点，上调miR-193 b可通过直接调控KRAS增加细胞凋亡率，抑制细胞增殖和迁移/侵袭。因此，我们的研究表明miR-193 b在人食管鳞癌的发生发展中起重要作用，可能成为未来食管鳞癌治疗的新靶点。
In recent years, microRNA-193b (miR-193b) is regarded as a tumor suppressor in the development and progression of various cancers. Several studies have indicated that KRAS could be regulated by miR-193b in pancreatic cancer cells. However, the function of miR-193b in human esophageal squamous cell carcinoma has not been explored intensively thus far. Herein, the relationship between miR-193b and KRAS was mainly explored in esophageal squamous cell carcinoma cells. In the present study, the expression levels of miR-193b and KRAS were assessed in both human esophageal cancer cells and tissues. The direct regulatory relationship between miR-193b and KRAS was evaluated using dual-luciferase assay. The effect of miR-193b overexpression and inhibitor on cell proliferation, migration/invasion, and apoptosis was further detected herein. Our results indicated that the expression of miR-193b was significantly lower in human esophageal cancer tissues than paracancerous tissues. The expression level of miR-193b/KRAS was stage-dependent in human esophageal cancers. KRAS was indicated as the direct target of miR-193b, and upregulation of miR-193b increased the percentage of cell apoptosis, and suppressed cell proliferation as well as cell migration/invasion via direct regulation of KRAS. Therefore, our study indicated that miR-193b plays an important role in the development and progression of human esophageal squamous cell carcinoma, which may become a novel target in the treatment of human esophageal squamous cell carcinoma in the future.