Optical diffusometry techniques and applications in biological agent detection
Optical diffusometry techniques and applications in biological agent detection
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DOI：
10.1115/1.2969430
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发表时间：
2008-11-01
影响因子：
2
通讯作者：
Wereley, Steve T.
中科院分区：
文献类型：
--
作者：
Kumar, Aloke;Gorti, Venu M.;Wereley, Steve T.
光学扩散测定法是用于测量扩散的技术。本工作探讨了直接测量亚微米颗粒物扩散系数用于病原体检测的可能性。这些颗粒的扩散系数是恒定温度下颗粒的阻力系数的函数。如果用适当的抗体功能化，则引入到含有分析物的样品中的颗粒与分析物结合。这导致颗粒的流体动力学阻力增加，因此其扩散系数降低。本研究利用上述原理，使用两种不同的实验方法有效地测量颗粒的扩散系数。所测量的扩散系数的降低可以与存在的分析物的量相关，并且因此形成生物制剂检测的基础。对实验条件的敏感性进行了分析。据观察，替代技术，如光阱持有承诺：光阱中的粒子的扩散行为被发现是定量不同的自由粒子。因此，先决条件确定，使光学陷阱适合代理检测。
Optical diffusometry is a technique used for measuring diffusion. This work explores the possibility of directly measuring diffusion coefficients of submicron particles for pathogen detection. The diffusion coefficient of these particles is a function of the drag coefficient of the particle at constant temperatures. Particles introduced into a sample containing an analyte bind with the analyte if functionalized with the appropriate antibodies. This leads to an increase in the hydrodynamic drag of the particles and hence a decrease in their diffusion coefficient. This study uses the above principle to effectively measure the diffusion coefficient of the particles using two different experimental approaches. The measured reduction in the diffusion coefficient can be correlated to the amount of analyte present and thus forms the basis of biological agent detection. Sensitivity to experimental conditions is analyzed. It is observed that alternative techniques such as optical trapping hold promise: the diffusive behavior of particles in optical traps is found to be quantitatively different from that of a free particle. Hence preconditions are identified to make optical trapping appropriate for agent detection.