Differential requirement for the function of SRD2, an snRNA transcription activator, in various stages of plant development
Differential requirement for the function of SRD2, an snRNA transcription activator, in various stages of plant development
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DOI：
10.1007/s11103-007-9271-7
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发表时间：
2008-02
影响因子：
5.1
通讯作者：
M. Ohtani;T. Demura;M. Sugiyama
中科院分区：
文献类型：
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作者：
M. Ohtani;T. Demura;M. Sugiyama
小核RNA （Small nuclear RNA, snRNA）是一类真核生物非编码RNA，在pre-mRNA剪接和rRNA加工中起着重要作用。由于这些功能是细胞活动的基础，snRNA转录的调控应该是所有真核生物的一个重要问题。本文通过分析拟南芥（Arabidopsis thaliana）中编码snRNA转录激活因子的thesrd2基因，阐述了snRNA转录的发育控制及其意义。在幼苗中，srd2在茎和根尖分生组织、叶片原基和根柱组织中表达水平较高，snRNA在这些组织中积累较多。在成熟植物中，srd2在发育中的叶片和花以及顶端分生组织中高度表达。srd2基因的突变干扰了srd2强表达区域发育的许多方面，但不是全部。值得注意的是，幼苗期顶端分生组织完全活跃状态的建立对rd2-1突变非常敏感，而已建立的分生组织的维持基本上不敏感。这些结果表明，在植物发育的不同阶段，对SRD2功能的需求是不同的。
Small nuclear RNA (snRNA) is a class of eukaryotic noncoding RNAs, which have essential roles in pre-mRNA splicing and rRNA processing. As these functions are fundamental to cell activities, the regulation of snRNA transcription should be a vital issue for all eukaryotes. Here we address developmental control of snRNA transcription and its significance through the analysis of theSRD2gene of Arabidopsis (Arabidopsis thaliana), which encodes an activator of snRNA transcription. In young seedlings, a high level ofSRD2expression was observed in shoot and root apical meristems, leaf primordia, and root stele tissues, where a large amount of snRNA accumulated. In mature plants,SRD2was highly expressed in developing leaves and flowers as well as apical meristems. Mutations in theSRD2gene interfered with many, but not all, aspects of development in the regions that showed strong expression ofSRD2. Of note, establishment of the fully active state of apical meristems in the seedling stage was very sensitive to thesrd2-1mutation, while maintenance of the established meristems was substantially insensitive. These results demonstrated differential requirement for the SRD2 function in various stages of plant development.