Solubilization of Expressed Proteins from Inclusion Bodies.
Solubilization of Expressed Proteins from Inclusion Bodies.
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包涵体表达蛋白的溶解。
DOI：
10.1101/pdb.prot102210
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发表时间：
2021
影响因子：
--
通讯作者：
Urbatsch,InaL
中科院分区：
文献类型：
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作者：
Kielkopf,ClaraL;Bauer,William;Urbatsch,InaL
外源蛋白在大肠杆菌中的高水平表达常常导致由表达蛋白的不溶性聚集体组成的细胞质颗粒或包涵体的形成。这些包涵体可以用相差显微镜观察到，并且很容易与大多数可溶性和膜结合的细菌蛋白分离，如本方案所述。
The expression of foreign proteins at high levels in Escherichia coli often results in the formation of cytoplasmic granules or inclusion bodies composed of insoluble aggregates of the expressed protein. These inclusion bodies can be seen with a phase-contrast microscope and are readily separated from most soluble and membrane-bound bacterial proteins, as described in this protocol.