Proteomic Analysis of Mamestra Brassicae Nucleopolyhedrovirus Progeny Virions from Two Different Hosts.
Proteomic Analysis of Mamestra Brassicae Nucleopolyhedrovirus Progeny Virions from Two Different Hosts.
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来自两种不同宿主的芸苔属核多角体病毒子代病毒颗粒的蛋白质组学分析。
DOI：
10.1371/journal.pone.0153365
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发表时间：
2016
期刊：
影响因子：
3.7
通讯作者：
Zhang LK
中科院分区：
文献类型：
--
作者：
Hou D;Chen X;Zhang LK
油菜夜蛾核型多角体病毒(MabrNPV)具有广泛的寄主范围，可在多种昆虫中复制。本研究利用已测序的MabrNPV株MabrNPV-CTA对棉铃虫和甜菜夜蛾两种寄主的BV和ODV进行了蛋白质组学分析。在ODV和BV中分别鉴定出82种和39种病毒蛋白。共鉴定出与ODV和BV相关的宿主蛋白分别为23和76个。病毒颗粒中的宿主蛋白主要参与细胞骨架、信号转导、囊泡运输、伴侣和代谢系统。一些宿主蛋白，如肌动蛋白、亲环素A和热休克蛋白70对病毒复制是重要的。在BV中还鉴定了几种参与免疫应答的宿主蛋白，其中C型凝集素蛋白首次被发现与BV相关，其家族成员已被证明参与了其他病毒的入侵过程。本研究为杆状病毒基因组的注释提供了方便，有助于我们了解杆状病毒的病毒粒子结构。此外，鉴定与体内产生的病毒粒子相关的宿主蛋白将有助于研究有趣的宿主蛋白在病毒复制中的参与。
Mamestra brassicae nucleopolyhedrovirus (MabrNPV) has a wide host range replication in more than one insect species. In this study, a sequenced MabrNPV strain, MabrNPV-CTa, was used to perform proteomic analysis of both BVs and ODVs derived from two infected hosts: Helicoverpa armigera and Spodoptera exigua. A total of 82 and 39 viral proteins were identified in ODVs and BVs, respectively. And totally, 23 and 76 host proteins were identified as virion-associated with ODVs and BVs, respectively. The host proteins incorporated into the virus particles were mainly involved in cytoskeleton, signaling, vesicle trafficking, chaperone and metabolic systems. Some host proteins, such as actin, cyclophilin A and heat shock protein 70 would be important for viral replication. Several host proteins involved in immune response were also identified in BV, and a C-type lectin protein was firstly found to be associated with BV and its family members have been demonstrated to be involved in entry process of other viruses. This study facilitated the annotation of baculovirus genome, and would help us to understand baculovirus virion structure. Furthermore, the identification of host proteins associated with virions produced in vivo would facilitate investigations on the involvement of intriguing host proteins in virus replication.