A Rsc3/Rsc30 zinc cluster dimer reveals novel roles for the chromatin remodeler RSC in gene expression and cell cycle control
A Rsc3/Rsc30 zinc cluster dimer reveals novel roles for the chromatin remodeler RSC in gene expression and cell cycle control
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DOI：
10.1016/s1097-2765(01)00219-2
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发表时间：
2001-04-01
期刊：
影响因子：
16
通讯作者：
Cairns, BR
中科院分区：
文献类型：
--
作者：
Angus-Hill, ML;Schlichter, A;Cairns, BR
染色质重塑复合体在转录调控中发挥核心作用。在这里，我们发现Rsc3和Rsc30是酵母重塑剂RSC复合物的新组成部分。Rsc3和Rsc30的功能需要它们的锌簇结构域，这是一个已知的位点特异性DNA结合基序。RSC3是必不可少的，rsc3ts突变体表现出涉及纺锤体组装检查点途径的G2/M细胞周期阻滞，而rsc30 δ突变体是活的和渗透敏感的。Rsc3和Rsc30作为稳定的RscS/Rsc30异质复合物在功能和物理上相互作用。然而，rsc3或rsc30突变体的DNA微阵列分析揭示了对核糖体蛋白基因和细胞壁基因表达水平的不同影响。我们认为Rsc3和Rsc30在物理上相互作用，但在靶向或调节RSC方面具有不同的作用。
Chromatin remodeling complexes perform central roles in transcriptional regulation. Here, we identify Rsc3 and Rsc30 as novel components of the essential yeast remodeler RSC complex. Rsc3 and Rsc30 function requires their zinc cluster domain, a known site-specific DNA binding motif. RSC3 is essential, and rsc3 Ts mutants display a G2/M cell cycle arrest involving the spindle assembly checkpoint pathway, whereas rsc30 Delta mutants are viable and osmosensitive. Rsc3 and Rsc30 interact functionally and also physically as a stable RscS/Rsc30 heteromeric complex. However, DNA microarray analysis with rsc3 or rsc30 mutants reveals different effects on the expression levels of ribosomal protein genes and cell wall genes. We propose that Rsc3 and Rsc30 interact physically but have different roles in targeting or regulating RSC.