Preparation of well-defined bovine polyhemoglobin based on dimethyl adipimidate and glutaraldebyde cross-linkage
Preparation of well-defined bovine polyhemoglobin based on dimethyl adipimidate and glutaraldebyde cross-linkage
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DOI：
10.1016/s0006-291x(02)00310-8
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发表时间：
2002-05-10
影响因子：
3.1
通讯作者：
Su, ZG
中科院分区：
文献类型：
--
作者：
Hu, T;Su, ZG
采用己二酰亚胺二甲酯（DMA）和戊二醛双交联法制备了结构明确的牛多血红蛋白。 DMA用于封闭血红蛋白的一些氨基，然后与戊二醛进一步聚合。 DMA与血红蛋白摩尔比为200时，血红蛋白的氨基修饰度为32%。戊二醛与DMA修饰的血红蛋白反应得到分子量分布窄（主要为128 kDa）的牛多血红蛋白。 DMA 修饰血红蛋白的 P-50 和 Hill 系数分别为 19.4 mm Hg 和 2.28，而牛多血红蛋白的 P-50 和 Hill 系数分别为 15.1 mm Hg 和 1.70。 DMA修饰的血红蛋白和多聚血红蛋白释放的玻尔质子数分别为0.86和0.56 H/四聚体。 (C) 2002 年爱思唯尔科学（美国）。版权所有。
A well-defined bovine polyhemoglobin was prepared by dimethyl adipimidate (DMA) and glutaraldehyde double cross-linkage method. DMA was used to block some amino groups of hemoglobin, followed by further polymerization with glutaraldehyde. The amino modification degree of hemoglobin was 32% when DMA reacted with hemoglobin at the molar ratio of 200. The bovine polyhemoglobin with narrow molecular weight distribution (mainly 128 kDa) was obtained when glutaraldehyde reacted with DMA-modified hemoglobin. The P-50 and the Hill coefficient for DMA-modified hemoglobin were 19.4 mm Hg and 2.28, respectively, while those for the bovine polyhemoglobin were 15.1 mm Hg and 1.70, respectively. The number of Bohr protons released for DMA-modified hemoglobin and the poly hemoglobin was 0.86 and 0.56 H/tetramer, respectively. (C) 2002 Elsevier Science (USA). All rights reserved.