Detection of hepatitis B virus markers using a biosensor based on imaging ellipsometry
Detection of hepatitis B virus markers using a biosensor based on imaging ellipsometry
复制标题
DOI：
10.1111/j.1365-2893.2009.01123.x
复制
发表时间：
2009-11-01
影响因子：
2.5
通讯作者：
Jin, G.
中科院分区：
文献类型：
--
作者：
Qi, C.;Zhu, W.;Jin, G.
一种基于成像椭偏仪（BIE）的生物传感器已被开发并在169例患者中验证，用于检测乙型肝炎病毒（HBV）感染的五种标志物。该方法的建立是为了为临床诊断铺平道路，包括配体筛选、灵敏度确定、临界值（cov）的建立以及与其他临床方法的比较。建立基质法筛选配体。利用受者工作特征曲线推导HBV标志物的cov。参照方法为酶联免疫吸附试验（ELISA）。选择生物活性高的配体，获得对乙型肝炎表面抗原（HBsAg）和表面抗体（anti-HBs）的敏感性分别为1 ng/mL和1 IU/mL。HBsAg、anti-HBs、乙型肝炎e抗原、乙型肝炎e抗体和核心抗体的cov分别为：15%、18%、15%、20%和15%，这是相应配体对照值的百分比。BIE可以在1小时内同时检测多达5种标记物，结果与ELISA一致，因此显示出高通量诊断乙型肝炎的潜力。
A biosensor based on imaging ellipsometry (BIE) has been developed and validated in 169 patients for detecting five markers of hepatitis B virus (HBV) infection. The methodology has been established to pave the way for clinical diagnosis, including ligand screening, determination of the sensitivity, set-up of cut-off values (CoVs) and comparison with other clinical methods. A matrix assay method was established for ligand screening. The CoVs of HBV markers were derived with the help of receiver operating characteristic curves. Enzyme-linked immunosorbent assay (ELISA) was the reference method. Ligands with high bioactivity were selected and sensitivities of 1 ng/mL and 1 IU/mL for hepatitis B surface antigen (HBsAg) and surface antibody (anti-HBs) were obtained respectively. The CoVs of HBsAg, anti-HBs, hepatitis B e antigen, hepatitis B e antibody and core antibody were as follows: 15%, 18%, 15%, 20% and 15%, respectively, which were the percentages over the values of corresponding ligand controls. BIE can simultaneously detect up to five markers within 1 h with results in acceptable agreement with ELISA, and thus shows a potential for diagnosing hepatitis B with high throughput.