Behavioural battery testing: Evaluation and behavioural outcomes in 8 inbred mouse strains
Behavioural battery testing: Evaluation and behavioural outcomes in 8 inbred mouse strains
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DOI：
10.1016/j.physbeh.2009.11.007
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发表时间：
2010-03-03
影响因子：
2.9
通讯作者：
Schalkwyk, Leonard Cornelis
中科院分区：
文献类型：
--
作者：
Lad, Heena Vanmalibhai;Liu, Lin;Schalkwyk, Leonard Cornelis
使用大规模行为电池来发现行为变异背后的新基因具有相当大的潜力。建立一个广泛的行为概况有助于更好地理解重叠遗传因素之间复杂的相互作用，从而形成各种范式，支撑系统生物学方法。我们设计了一系列的测试来剖析和描述行为表型的遗传基础，但首先要评估几个方面，这些方面被认为可能会混淆定量性状的定位。其中包括调查：个人与兄弟姐妹住房；在一天中的不同时间进行测试；电池与非电池测试；初始位置在明暗相间的盒子里。此外，我们评估了8个自交系的电池行为特征的差异。总体而言，我们发现行为电池对成对住房效应最为敏感，其中在开阔场地，高架迷宫和光暗箱中的体重和某些措施在兄弟姐妹饲养的小鼠和单独饲养的小鼠之间存在显着差异。在一天中的不同时间测试以及电池与非电池测试中，几乎没有发现大的影响。置于明暗箱中会影响活动和持续时间，从而深刻影响分析结果。8个自交系（C57BL/6J、129S1/SvImJ、A/J、BALB/cByJ、C3H/HeJ、DBA/2J、FVB/NJ和SJL/J）的行为特征在野外和光暗试验中表现出了显著的品系排序差异。然而，一些测试，如高架加迷宫，在不同的测量中产生了不协调的应变排序效应。本文报道的研究结果证实了行为电池用于绘制小鼠参考种群中自然发生的变异的前景。(C) 2009爱思唯尔公司版权所有。
The use of large scale behavioural batteries for the discovery of novel genes underlying behavioural variation has considerable potential. Building a broad behavioural profile serves to better understand the complex interplay of overlapping genetic factors contributing to various paradigms, underpinning a systems biology approach. We devised a battery of tests to dissect and characterise the genetic bases of behavioural phenotypes, but firstly undertook to evaluate several aspects considered potentially confounding for mapping quantitative traits. These included investigating: individual versus sibling housing; testing at different times during the day; battery versus non-battery testing; and initial placement within the light-dark box. Furthermore, we assessed how behavioural profiles differed in our battery across 8 inbred strains. Overall, we found the behavioural battery was most sensitive to paired-housing effects, where weight and some measures in the open field, elevated plus maze and light-dark box differed significantly between sibling housed and singly housed mice. Few large effects were found for testing at different times of day and battery versus non-battery testing. Placement in the light-dark box influenced activity and duration measures, which profoundly affected the analysis outcome. Behavioural profiles across eight inbred strains (C57BL/6J, 129S1/SvImJ, A/J, BALB/cByJ, C3H/HeJ, DBA/2J, FVB/NJ, and SJL/J) demonstrated some robust strain ranking differences for measures in the open field and light-dark tests in our battery. However, some tests such as the elevated plus maze produced incongruous strain ranking effects across measures. The findings reported herein bear out the promise of behavioural batteries for mapping naturally occurring variation in mouse reference populations. (C) 2009 Elsevier Inc. All rights reserved.