Natural killing and antibody-dependent cellular cytotoxicity are independent immune functions in the Minnesota miniature swine.
Natural killing and antibody-dependent cellular cytotoxicity are independent immune functions in the Minnesota miniature swine.
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自然杀伤和抗体依赖性细胞毒性是明尼苏达小型猪的独立免疫功能。
DOI：
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发表时间：
1978
影响因子：
11.1
通讯作者：
Y. B. Kim
中科院分区：
文献类型：
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作者：
H. Koren;D. Amos;Y. B. Kim
明尼苏达小型猪外周血淋巴细胞进行了测试自然杀伤（NK）和抗体依赖性细胞毒性（ADCC）在2至4小时51铬释放试验对人骨髓和淋巴肿瘤靶细胞。在两种试验中，成年无特定病原体和无菌动物均表现出正常水平的活性。此外，还测定了缺初乳仔猪外周血淋巴细胞的NK和ADCC活性。这些动物是通过子宫切除术获得的，并且先前显示为免疫学上的“处女”。“我们发现这些新生仔猪表现出正常的ADCC，但缺乏NK活性。这两种活动的个体发育差异表明它们是不同的。初步效应细胞表征研究表明：（i）猪中的NK和ADCC在物理上不可分离;（ii）基于细胞/细胞的大部分细胞毒性活性由非T淋巴细胞部分介导;（iii）玫瑰花状T细胞（约占猪外周血淋巴细胞总数的60%）也具有较低但可证实的细胞毒性活性。
Peripheral blood lymphocytes from Minnesota miniature pigs were tested for natural killing (NK) and antibody-dependent cellular cytotoxicity (ADCC) in a 2- to 4-hr 51Cr release assay against human myeloid and lymphoid tumor target cells. Adult specific pathogen-free and germfree animals exhibited normal levels of activity in both assays. In addition, the NK and ADCC activities of peripheral blood lymphocytes from colostrum-deprived newborn piglets were examined. These animals were obtained by hysterectomy and previously shown to be immunologically "virgin." We found that these newborn piglets exhibited normal ADCC but lacked NK activity. The differences in the ontogeny of the two activities suggest that they are distinct. Preliminary effector cell characterization studies suggest that: (i) NK and ADCC in the pig are physically not separable; (ii) the majority of the cytotoxic activity on a cell-per-cell basis is mediated by the non-T lymphocyte fraction; and (iii) the rosetted T cells, which account for about 60% of the total pig peripheral blood lymphocytes, have low but demonstrable cytotoxic activity as well.