REPAIR OF 8‐METHOXYP SORALEN PHOTOINDUCEDCROSS‐LINKS and MUTAGENESIS: ROLE OF THE DIFFERENT REPAIR PATHWAYS IN YEAST
REPAIR OF 8‐METHOXYP SORALEN PHOTOINDUCEDCROSS‐LINKS and MUTAGENESIS: ROLE OF THE DIFFERENT REPAIR PATHWAYS IN YEAST
复制标题
8-甲氧基索拉林光诱导交联的修复和诱变：不同修复途径在酵母中的作用
DOI：
10.1111/j.1751-1097.1985.tb03487.x
复制
发表时间：
1985
影响因子：
3.3
通讯作者：
E. Moustacchi
中科院分区：
文献类型：
--
作者：
C. Cassier;R. Chanet;E. Moustacchi
摘要：在酿造酵母中，用8 -甲氧基补骨脂素（8 - MOP）预处理后，用饱和剂量的UVA再照射，加上低剂量的UVA和去除未结合的8 - MOP，导致在切除（radl‐3,radl‐Δ， rad2‐6）或重组（rad52‐1）修复途径中有缺陷的菌株中正向突变的频率增加。在诱变修复途径阻断的突变体（rad6‐Δ和pso2‐1）中，没有观察到这种由DNA链间交联引起的增强。这些结果被解释为揭示了DNA交联切除的替代途径的存在。这种途径似乎容易出错，并且独立于重组途径。RAD6或PSO2基因产物可能会干扰这一过程。
Abstract— In Saccharomyces cerevisiae, a re‐irradiation with a saturing dose of UVA after pretreatment with 8‐methoxypsoralen (8‐MOP) plus low doses of UVA and removal of unbound 8‐MOP lead to an increase in frequency of forward mutants in strains defective in the excision (radl‐3, radl‐Δ, rad2‐6) or in the recombinational (rad52‐l) repair pathways. Such an enhancement attributable to DNA interstrand cross‐links was not observed in mutants blocked in a mutagenic repair pathway (rad6‐Δ and pso2‐l). These results are interpreted as revealing the existence of an alternative pathway to excision of DNA cross‐links. This pathway appears to be error‐prone and independent from the recombinational pathway. The RAD6 or the PSO2 gene products are likely to interfere with this process.