The xCELLigence System for Real-Time and Label-Free Monitoring of Cell Viability
The xCELLigence System for Real-Time and Label-Free Monitoring of Cell Viability
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DOI：
10.1007/978-1-61779-108-6_6
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发表时间：
2011-01-01
期刊：
影响因子：
--
通讯作者：
Abassi, Yama A.
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文献类型：
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作者：
Ke, Ning;Wang, Xiaobo;Abassi, Yama A.
我们在这里描述了使用xCELLigence系统进行无标记和实时监测细胞活力。XCELLigence系统使用专门设计的含有交错金微电极的微滴定板，以电阻抗作为读数，非侵入性地监测培养细胞的活力。通过xCELLigence系统对细胞活力的持续监测，可以区分细胞活力的不同扰动，如衰老、细胞毒性(细胞死亡)和增殖减少(细胞周期停滞)。此外，xCELLigence系统的时间分辨率允许确定执行标准细胞存活率分析的最佳时间点以及了解作用模式的其他终点分析。我们使用了WST-1试验(终点活性读数)、细胞指数测定(xCELLigence连续监测活性)和DNA片段化试验(终点凋亡试验)，系统地检测了两种不同细胞杀伤动力学的细胞毒性化合物所引发的细胞毒效应。WST-1细胞活力读数与细胞指数有很好的相关性。XCELigence读数的时间分辨率的重要性体现在它能够精确定位进行终点存活率和细胞凋亡检测的最佳时间点。
We describe here the use of the xCELLigence system for label-free and real-time monitoring of cell viability. The xCELLigence system uses specially designed microtiter plates containing interdigitated gold microelectrodes to noninvasively monitor the viability of cultured cells using electrical impedance as the readout. The continuous monitoring of cell viability by the xCELLigence system makes it possible to distinguish between different perturbations of cell viability, such as senescence, cell toxicity (cell death), and reduced proliferation (cell cycle arrest). In addition, the time resolution of the xCELLigence system allows for the determination of optimal time points to perform standard cell viability assays as well as other end-point assays to understand the mode of action. We have used the WST-1 assay (end-point viability readout), the cell index determination (continuous monitoring of viability by xCELLigence), and the DNA fragmentation assay (end-point apoptosis assay) to systematically examine cytotoxic effects triggered by two cytotoxic compounds with different cell-killing kinetics. Good correlation was observed for viability readouts between WST-1 and cell index. The significance of time resolution by xCELLigence readout is exemplified by its ability to pinpoint the optimal time points for conducting end point viability and apoptosis assays.