Cold tolerance response mechanisms revealed through comparative analysis of gene and protein expression in multiple rice genotypes
Cold tolerance response mechanisms revealed through comparative analysis of gene and protein expression in multiple rice genotypes
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DOI：
10.1371/journal.pone.0218019
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发表时间：
2019-06-10
期刊：
影响因子：
3.7
通讯作者：
Pereira, Andy
中科院分区：
文献类型：
--
作者：
de Freitas, Gabriela Moraes;Thomas, Julie;Pereira, Andy
由于其热带起源和适应性，水稻受到冷胁迫的严重影响，因此在生长和生产力方面遭受巨大损失。目前，稻米是世界上消费量第二大的谷物，在“重大气候变化”背景下，极端气温事件造成的产量损失可能对世界经济产生重大影响。本文通过对低温胁迫下水稻基因型的分析，从生理气体交换参数、光合色素和抗氧化剂的生化变化以及基因和蛋白表达水平等方面，探讨了水稻耐低温的多种机制。低温胁迫对各基因型光合作用的影响均表现为第一效应。然而，热带粳稻基因型Secano做巴西和柏树有一个更大的减少气体交换参数，如光合作用和水分利用效率相比，温带粳稻日本晴和M202基因型。生化分析表明，尽管耐低温植物的影响，他们迅速调整，以维持其细胞内环境稳定的抗氧化剂和渗透压物质，如酚类化合物和脯氨酸的积累。耐冷基因型和敏感基因型在OsGH 3 -2、OsSRO 1a、OsZFP 245和OsTPP 1的转录水平的基因表达以及LRR-RLK、bHLH、GLYI和LTP 1蛋白的蛋白水平的表达上显示出明显的差异。本研究通过对日本晴和M202基因型的生理生化反应及相关基因和蛋白表达谱的分析，阐明了日本晴和M202基因型的耐寒性特征。显示差异表达反应的基因和蛋白质是理解水稻中受影响的生物学途径和工程耐冷性的显着候选者，以产生能够在冷胁迫下维持生长、发育和繁殖的品种。我们还提出，分析的基因的作用机制与耐受性反应。
Due to its tropical origin and adaptation, rice is significantly impacted by cold stress, and consequently sustains large losses in growth and productivity. Currently, rice is the second most consumed cereal in the world and production losses caused by extreme temperature events in the context of "major climatic changes" can have major impacts on the world economy. We report here an analysis of rice genotypes in response to low-temperature stress, studied through physiological gas-exchange parameters, biochemical changes in photosynthetic pigments and antioxidants, and at the level of gene and protein expression, towards an understanding and identification of multiple low-temperature tolerance mechanisms. The first effects of cold stress were observed on photosynthesis among all genotypes. However, the tropical japonica genotypes Secano do Brazil and Cypress had a greater reduction in gas exchange parameters like photosynthesis and water use efficiency in comparison to the temperate japonica Nipponbare and M202 genotypes. The analysis of biochemical profiles showed that despite the impacts of low temperature on tolerant plants, they quickly adjusted to maintain their cellular homeostasis by an accumulation of antioxidants and osmolytes like phenolic compounds and proline. The cold tolerant and sensitive genotypes showed a clear difference in gene expression at the transcript level for OsGH3-2, OsSRO1a, OsZFP245, and OsTPP1, as well as for expression at the protein level for LRR-RLKs, bHLH, GLYI, and LTP1 proteins. This study exemplifies the cold tolerant features of the temperate japonica Nipponbare and M202 genotypes, as observed through the analysis of physiological and biochemical responses and the associated changes in gene and protein expression patterns. The genes and proteins showing differential expression response are notable candidates towards understanding the biological pathways affected in rice and for engineering cold tolerance, to generate cultivars capable of maintaining growth, development, and reproduction under cold stress. We also propose that the mechanisms of action of the genes analyzed are associated with the tolerance response.