Hybrid de novo genome assembly of Chinese chestnut (Castanea mollissima)
Hybrid de novo genome assembly of Chinese chestnut (Castanea mollissima)
复制标题
板栗杂交基因组从头组装（Castanea mollissima）
DOI：
10.1093/gigascience/giz112
复制
发表时间：
2019-09-01
期刊：
影响因子：
9.2
通讯作者：
Qin, Ling
中科院分区：
文献类型：
--
作者：
Xing, Yu;Liu, Yang;Qin, Ling
背景：板栗（Castanea mollissima）在中国广泛种植，用于生产坚果。该植物在造林和生态系统服务方面也具有重要的生态作用。为了促进和扩大软软菇的育种和遗传改良，我们在这里报道了软软菇的全基因组序列。研究结果：我们利用太平洋生物科学公司的单分子测序技术获得了高质量的C. mollissima基因组图谱。最终的草图基因组长785.53 Mb，连续N50大小为944 kb，我们进一步注释了基因组中的36,479个蛋白质编码基因。系统发育分析表明，C. mollissima起源于壳斗科成员Quercus robur，距今约1362万年前。结论：高质量的核桃全基因组图谱将为核桃抗病育种和遗传改良提供宝贵资源。
Background: The Chinese chestnut (Castanea mollissima) is widely cultivated in China for nut production. This plant also plays an important ecological role in afforestation and ecosystem services. To facilitate and expand the use of C. mollissima for breeding and its genetic improvement, we report here the whole-genome sequence of C. mollissima. Findings: We produced a high-quality assembly of the C. mollissima genome using Pacific Biosciences single-molecule sequencing. The final draft genome is similar to 785.53 Mb long, with a contig N50 size of 944 kb, and we further annotated 36,479 protein-coding genes in the genome. Phylogenetic analysis showed that C. mollissima diverged from Quercus robur, a member of the Fagaceae family, similar to 13.62 million years ago. Conclusions: The high-quality whole-genome assembly of C. mollissima will be a valuable resource for further genetic improvement and breeding for disease resistance and nut quality.