Long-term impacts of zinc and copper enriched sewage sludge additions on bacterial, archaeal and fungal communities in arable and grassland soils
Long-term impacts of zinc and copper enriched sewage sludge additions on bacterial, archaeal and fungal communities in arable and grassland soils
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DOI：
10.1016/j.soilbio.2011.01.004
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发表时间：
2011-05-01
影响因子：
9.7
通讯作者：
McGrath, Steve P.
中科院分区：
文献类型：
--
作者：
Macdonald, Catriona A.;Clark, Ian M.;McGrath, Steve P.
来自污水污泥的金属污染对土壤微生物群落的长期影响已被广泛评估，但混杂效应使得难以得出确切的结论，从而难以就安全金属限值提出建议。在这里，我们使用多重末端限制性长度片段多态性（M-TRFLP），以评估长期的影响，污泥携带的锌和铜污染的细菌，真菌和古菌群落的结构在7个不同的土壤在金属水平相关的当前指南限制。尽管地点对微生物群落结构有很强的影响，但相似性分析（ANOSIM）表明锌对细菌（P < 0.001）、古细菌（P < 0.001）和真菌（P < 0.001）有很小但显着的影响。铜对细菌、古菌和真菌的影响也极显著（P <0.001）。几种细菌和真菌T-RF被鉴定为响应Zn或Cu。如细菌T-RF 72与Zn和Cu呈负相关，T-RF 259与Zn呈正相关。试图确定这些细菌标志物的锌和铜污染表明铜对酸性细菌在耕地土壤中的负面影响。这些结果表明，第一次，尽管网站上的微生物群落结构的强烈影响，锌和铜来自污水污泥的影响，可以检测到的细菌，真菌和古菌群落的变化，表明一个共同的反应超过11年后，污泥添加。(C)2011爱思唯尔有限公司版权所有。
Long-term impacts of metal contamination derived from sewage sludge on soil microbial communities have been widely evaluated, but confounding effects have made it difficult to draw firm conclusions and thus to advise on safe metal limits. Here we used Multiplex-terminal restriction length fragment polymorphism (M-TRFLP) to assess the long-term impact of sludge-borne Zn and Cu contamination on the structure of bacterial, fungal and archaeal communities across seven different soils at metal levels relevant to current guideline limits. Despite strong effects of site on microbial community structure, analysis of similarity (ANOSIM) demonstrated a small but significant effect of Zn on bacteria (P < 0.001), archaea (P < 0.001), and fungi (P < 0.001). Significant effects of Cu on bacteria (P < 0.001), archaea (P < 0.001) and fungi (P < 0.001) were also observed. Several bacterial and fungal T-RFs were identified as responding to Zn or Cu. For example the bacterial T-RF 72 was negatively correlated with Zn and Cu, and T-RF 259 was positively correlated with Zn. Attempts to identify these bacterial markers of Zn and Cu contamination suggest a negative impact of Cu on Acidobacteria in arable soils. These results demonstrate for the first time, that despite a strong influence of site on microbial community structure, effects of Zn and Cu derived from sewage sludge can be detected as shifts in bacterial, fungal and archaeal communities indicating a common response more than 11 years after sludge addition. (C) 2011 Elsevier Ltd. All rights reserved.