Continuous detection of extracellular ATP on living cells by using atomic force microscopy.
Continuous detection of extracellular ATP on living cells by using atomic force microscopy.
复制标题
DOI：
10.1073/pnas.96.21.12180
复制
发表时间：
1999-10
影响因子：
11.1
通讯作者：
Stefan W. Schneider;Marie E. Egan;B. Jena;W. Guggino;Hans Oberleithner;J. P. Geibel
中科院分区：
文献类型：
--
作者：
Stefan W. Schneider;Marie E. Egan;B. Jena;W. Guggino;Hans Oberleithner;J. P. Geibel
原子力显微镜是一种强大的技术，用于研究生理条件下活细胞的表面。仪器的分辨率主要受到活细胞的柔软度以及与扫描尖端（悬臂）的相互作用的限制。原子力显微镜与肌球蛋白功能化悬臂相结合，用于检测溶液中和活细胞上的 ATP 浓度。使用功能活性吸头扫描培养物中的细胞表面，并显示 CFTR+ 细胞系 (S9) 具有可用原子力显微镜检测到的基底表面 ATP 浓度 (n = 10)。在使用未包被或热灭活酶包被吸头扫描的细胞中检测不到 ATP 依赖性信号 (n = 9)。酶活性尖端可以作为原子力显微镜生物传感器未来开发的模型，该传感器可以同时检测活细胞表面微环境中的地形和生物学重要化合物。
Atomic force microscopy is a powerful technique used to investigate the surface of living cells under physiological conditions. The resolution of the instrument is mainly limited by the softness of living cells and the interactions with the scanning tip (cantilever). Atomic force microscopy, in combination with myosin-functionalized cantilevers, was used in the detection of ATP concentrations in solution and on living cells. Functionally active tips were used to scan the surface of cells in culture and to show that the CFTR+ cell line (S9) had a basal surface ATP concentration that could be detected with atomic force microscopy (n = 10). ATP-dependent signals were not detectable in cells scanned with noncoated or heat-inactivated enzyme-coated tips (n = 9). Enzymatically active tips may serve as a model for future development of atomic force microscopy biosensors that can simultaneously detect topographical and biologically important compounds at the surface microenvironment of living cells.