Protein coingestion stimulates muscle protein synthesis during resistance-type exercise
Protein coingestion stimulates muscle protein synthesis during resistance-type exercise
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DOI：
10.1152/ajpendo.00774.2007
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发表时间：
2008-07-01
影响因子：
5.1
通讯作者：
van Loon, Luc J. C.
中科院分区：
文献类型：
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作者：
Beelen, Milou;Koopman, Rene;van Loon, Luc J. C.
与营养干预对运动后肌肉蛋白质合成的影响相反，人们对运动期间调节蛋白质合成的潜力知之甚少。本研究探讨了阻力型运动期间蛋白质与碳水化合物的共同摄入对肌肉蛋白质合成的影响。十名健康男性在全天实行标准化饮食后，在晚上进行了研究。受试者参加了两项实验，在 2 小时的阻力运动过程中，他们摄入碳水化合物或碳水化合物和蛋白质。受试者在运动前和运动期间每 15 分钟接受一次测试饮料，剂量为 0.15 克。千克（-1）。 h(-1) 碳水化合物，含 (CHO + PRO) 或不含 (CHO) 0.15 g。千克（-1）。 h(-1) 蛋白质水解产物。连续静脉输注L-[环-C-13(6)]苯丙氨酸和L-[环-H-2(2)]酪氨酸，并收集血液和肌肉活检以评估运动期间的全身和肌肉蛋白质合成率。与仅摄入碳水化合物相比，同时摄入蛋白质可使全身蛋白质分解率降低 8.4 +/- 3.6% (P = 0.066)，并使蛋白质氧化和合成率分别提高 77 +/- 17 和 33 +/- 3% (P < 0.01)。因此，CHO 中全身净蛋白质平衡为负，而 CHO + PRO 处理后实现正净平衡（分别为 -4.4 +/- 0.3 与 16.3 +/- 0.4 μmol 苯丙氨酸.kg(-1).h(-1)；P < 0.01）。相应地，蛋白质共摄取后混合肌肉蛋白分数合成率高出 49 +/- 22%（CHO + PRO 与 CHO 处理分别为 0.088 +/- 0.012 和 0.060 +/- 0.004%/h；P < 0.05）。我们的结论是，即使在进食状态下，蛋白质共同摄入也会在阻力型运动期间刺激全身和肌肉蛋白质合成率。
In contrast to the effect of nutritional intervention on postexercise muscle protein synthesis, little is known about the potential to modulate protein synthesis during exercise. This study investigates the effect of protein coingestion with carbohydrate on muscle protein synthesis during resistance-type exercise. Ten healthy males were studied in the evening after they consumed a standardized diet throughout the day. Subjects participated in two experiments in which they ingested either carbohydrate or carbohydrate with protein during a 2-h resistance exercise session. Subjects received a bolus of test drink before and every 15 min during exercise, providing 0.15 g . kg(-1) . h(-1) carbohydrate with (CHO + PRO) or without (CHO) 0.15 g . kg(-1) . h(-1) protein hydrolysate. Continuous intravenous infusions with L-[ring-C-13(6)] phenylalanine and L-[ring-H-2(2)] tyrosine were applied, and blood and muscle biopsies were collected to assess whole body and muscle protein synthesis rates during exercise. Protein coingestion lowered whole body protein breakdown rates by 8.4 +/- 3.6% (P = 0.066), compared with the ingestion of carbohydrate only, and augmented protein oxidation and synthesis rates by 77 +/- 17 and 33 +/- 3%, respectively (P < 0.01). As a consequence, whole body net protein balance was negative in CHO, whereas a positive net balance was achieved after the CHO + PRO treatment (-4.4 +/- 0.3 vs. 16.3 +/- 0.4 mu mol phenylalanine . kg(-1) . h(-1), respectively; P < 0.01). In accordance, mixed muscle protein fractional synthetic rate was 49 +/- 22% higher after protein coingestion (0.088 +/- 0.012 and 0.060 +/- 0.004%/ h in CHO + PRO vs. CHO treatment, respectively; P < 0.05). We conclude that, even in a fed state, protein coingestion stimulates whole body and muscle protein synthesis rates during resistance-type exercise.